雌激素受体和细胞增殖周期调控蛋白D1在分化型甲状腺癌中的表达及意义

目的:探讨雌激素受体(ER)和细胞增殖周期调控蛋白(cyclinD1)在分化型甲状腺癌(DTC)中的表达及意义。方法:用免疫组织化学SP法研究不同甲状腺组织中ER和cyclinD1的表达。DTC43例(乳头状癌39例,滤泡状癌4例),甲状腺良性腺瘤30例,腺瘤旁正常组织16例。结果:DTC组织中ER和cyclinD1蛋白阳性率分别为53.5%(23/43)、65.1%(28/43);甲状腺良性瘤中ER和cyclinD1蛋白阳性率分别为26.7%(8/30)、36.7%(11/30);正常甲状腺组织中ER阳性率为12.5%(2/16),而cyclinD1则不表达。DTC中ER的表达明显高于甲状腺良性腺瘤和正常甲状腺组织(P<0.05);DTC中cyclinD1的表达明显高于甲状腺良性腺瘤(P<0.05);且DTC中cyclinD1和ER表达存在正相关关系(P<0.05)。结论:雌激素在DTC的发生、发展中有促进细胞增殖的作用;DTC可能为雌激素依赖性肿瘤。

Expression of the estrogen receptor and Cyclin D1 in differentiated thyrord carcinoma and its clinical significance

Purpose:To investigate the expression and clinical significance of estrogen receptor and Cyclin D1 in the differentiated thyrord carcinoma. Methods:We investigated the expression of ER and Cyclin D1 in thyroid normal tissue, thyroid adenoma and DTC by SP immunohistochemical technique. We have collected 89 specimens from surgical resections, and all the sections were formalin-fixed and paraffin-embedded, each has detailed clinical data, two pathologists classified the histopathologic types. DTC 43 cases (papillary thyroid carcinoma, 39 cases, follicular thyroid carcinoma, 4 cases); thyroid follicular adenoma 30 cases, and 16 cases of normal thyroid tissues taken from around the thyroid adenoma. Results:The positive rates of ER and CyclinD1 in the DTC were 53.5%(23/43), 65.1%(28/43) respectively; the positive rates of ER and CyclinD1 in the thyroid adenoma were 26.7%(8/30), 36.7%(11/30) respectively; the positive rates of ER in normal thyroid tissues were 12.5%(2/16), while CyclinD1 were negative. The ER expression in DTC was significantly higher than that in the thyroid adenoma and the normal thyroid tissues(P<0.05) ; the CyclinD1 expression in DTC was significantly higher than that in the thyroid adenoma(P<0.05); There was significant positive correlation between the CyclinD1 expression and the ER expression(P<0.05). Conclusions:Estrogen may play a role in accelerating proliferation in the occurrence and development of the DTC. It is likely that DTC are estrogen-dependent tumors.