CYP1A1与GSTM1的多态性与原发性肝癌遗传易感性的关系研究

目的 :探讨细胞色素P4 5 0 1A1(cytochromeP4 5 0 1A1,CYP1A1)与谷胱苷肽S -转移酶M1(glu tathioneS transferaseM 1,GSTM 1)的多态性与原发性肝癌遗传易感性的关系。方法 :应用等位特异PCR和多重PCR技术对 5 2例原发性肝癌患者和 10 0名健康对照的CYP1A1和GSTM 1多态性进行分析。结果 :肝癌患者CYP1A1第 7外显子 4 62Val的等位变异频率为 0 .4 6,显著高于正常对照的变异频率(0 .2 2 ) ,病例组GSTM1的纯合型缺失频率 (0 .65 )也显著高于对照组 (0 .4 1) ,携带有CYP1A1Val/Val纯合变异和GSTM1纯合缺失基因型的人患肝癌的风险大大增加 ,前者的比值比 (oddsratio ,OR)及 95 %可信区间 (confidenceinterval,95 %CI)为 4 .13(1.2 8～ 13.35 ) ,后者的OR值及 95 %CI为 2 .72 (1.35～5 .4 6) ,二者联合OR值及 95 %CI为 8.5 0 (1.74～ 4 1.5 0 )。结论 :CYP1A1和GSTM 1的多态是原发性肝癌的遗传易感因素 ,二者的等位变异增加了患肝癌的风险

Relationship Study Between Gene Polymorphism of CYP1A1,GSTM1 and Genetic Susceptibility of Primary Hepatocellar Carcinoma

Objective To study relationship between gene polymorphism of CYP1A1?GSTM1 and genetic susceptibility of primary hepatocellar carcinoma.Methods Polymorphism of CYP1A1 and GSTM1 was analyzed in 52 patients with primary hepatocellar carcinoma and 100 health controls by AS PCR and MD PCR.Results The allele frequency of CYP1A1 exon7 Va1 and GSTM1 in patents with primary hepatocellar cancinoma was found to be 0.46 and 0.65,which was significently higher than 0.22 and 0.41 of controls.The odds ratios for CYP1A1Val/Val genotype?GSTM1 nu11 genotype and both genotypes combined were 4.13 (1.28-13.35) and 2.72 (1.35-5.46) and 8.50(1.74-41.50),respectively.Conclusion The study suggest that individuals who have CYP1A1 and GSTM1 variant allele might be at higher risk for developing primary hepatocellar carcinoma.