| 菊三七的组织培养与快速繁殖研究 |
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| 引用本文： | 吴斌,吴德康,陈坚波.菊三七的组织培养与快速繁殖研究[J].时珍国医国药,2004,15(4):252-253. |
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| 作者姓名： | 吴斌 吴德康 陈坚波 |
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| 作者单位： | 1. 南京中医药大学,江苏,南京,210029
2. 金华职业技术学院医学院,浙江,金华,321000 |
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| 摘 要： | 目的:在菊三七自然繁殖率低下的情况,探讨利用组织培养方法解决繁殖的问题。方法:不同部位菊三七外植体进行无菌苗的诱导培养。结果:诱导率表现为茎尖>带腋芽的茎段,而叶片则不能被诱导发芽。结论:茎尖是理想的快速繁殖材料。初代培养过程中,MS 6BA(2mg/L) NAA(0.2mg/L)为最佳培养基;MS NAA(0.2mg/L)为理想的继代增殖培养基;1/2MS IBA(1.0mg/L) NAA(0.1mg/L)为最佳的生根培养基。
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| 关 键 词： | 菊三七 组织培养 快速繁殖 中药 植物药 |
| 文章编号： | 1008-0805(2004)04-0252-01 |
| 修稿时间： | 2003年8月12日 |
Tissue Culture and Rapid Propagation of Gynura segetum |
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| WU Bin,WU De-kang,CHEN Jian-bo.Tissue Culture and Rapid Propagation of Gynura segetum[J].Lishizhen Medicine and Materia Medica Research,2004,15(4):252-253. |
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| Authors: | WU Bin WU De-kang CHEN Jian-bo |
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| Institution: | WU Bin~1,WU De-kang~1,CHEN Jian-bo~2 |
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| Abstract: | Objective:To make it possible that Gynura segetum can be rapid propagation. Methods: The different parts of explant of Gynura segetum were made induced culture. Results: Inductive rate presents stem terminal bud auxiliary bud, but leaves can not be budded off. Conclusion: In tbe process of subculture, the subculture effect of culture medium MS NAA (0.2mg/L) is tile best. Culture medium of root initiation 1/2MS IBA(1.0mg/L) NAA(0.1mg/L) have the best effect. |
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| Keywords: | Gynura segetum Tissue culture Rapid propagation |
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