胃粘膜异型增生的PCNA、P53和C-erbB-2表达及其诊断价值

 应用免疫组化SP方法, 对65例异型增生胃粘膜PCNA(PC10)、CerbB-2(PAb1)和P53蛋白进行了测定。胃粘膜异型增生Ⅰ级PCNA平均指数22.34%, Ⅱ级32.58%, Ⅲ级46.08%, 各组间比较差异有显著性(P＜0.01), PCNA平均指数与异型增生病理分级间有很好地相关性(r＝0.966, P＜0.0005); Ⅰ、Ⅱ级异型增生无P(53)和C－erbB－2蛋白表达, Ⅲ级中P(53)蛋白阳性8例(32%), C-erbB-2蛋白阳性7例(28%)。 本文还就PCNA指数和P(53)、C－erbB－2蛋白表达的意义进行了讨论。

Expression and their diagnostic value of proliferating Cell nuclear antigen (PCNA)、P53 and C-erbB-2 proteins in dysplastic gastri mucosa

The SP immunohistochemical method was performed On 65 cases of dysplastic gastric mucosa to detect expression of PCNA (PC10). P53 (Do-7) and C-erbB-2 (PAb1) proteins. The results showed that the mean PCNA indices were 22.34% ,32. 58 % and 46 .08 % respectively, and there was a significant difference (P＜0.01)between these Varied groups, the mean PCNA index was increased with the progression of the pathologic grade in dysplastic gastric mucosa, there were significantly positive correlation (r=0.966, P=0.0005) between the pathologic grade andPCNA index; Abnormal expression of C-erbB-2 and P53 proteins were not observedin grade Ⅰ and Ⅱ dysplastic gastrlc mucosa, While C-erbB-2 was found in 7 of the 25 grade Ⅲ (28%), and P⁵³ in 8 of the 25 grade Ⅲ (32%) dysplastic gastric mucosarespectively. The signlficance on expression of PCNA index, P53 and C-erbB-2 proteins are also discussed in dysplastic gastric mucosa.