PCR-SSCP分析法检测宫颈癌中H-ras、p53基因突变与HPV感染

 我们对40例宫颈癌活检组织同时进行HPV、H－ras、p53基因的检测，应用GP－PCR技术检测了多型HPV的感染，发现阳性率为85％（34／40）。HPV16转化基因E7占61．8％（21／34）。此外，利用PCR－SSCP（单纯DNA构象多态性）分析方法，对宫颈癌中H－ras、p53基因点突变进行了检测，H－ras突变占22．5％（9／40），p⁵³占2．5％（1／40），而H－ras突变在HPV感染阳性组织中占14．7％（5／34），其中3例的病程分期为Ⅲ期以上。结果表明：HPV16E7转化基因在宫颈癌的致癌过程中为关键基因，H－ras突变很可能与有关报道相一致，属于“迟发”事件，与肿瘤的恶性程度及浸润有关。而p⁵³5—6外显子基因突变在HPV感染的宫颈癌中发生率很低。

Detection of p~(53) H-ras Gene Mutations by PCR-SSCP analysis and Human Papillomavirus Infection in Cervical Carcinoma

p⁵³、H-ras gene mutations and human papillomavirus infection were detected in 40 speciments of cervical carcinoma.GP-PCR (general primers-PCR) was applied to testing for various HPV infection.The positive rate was 85 0 o (34/40).The incidence of HPV16 E7 transformed gene was 61·800(21/34)·Otherwise，H-ras、p⁵³ gene mutation were detected by PCR-SSCP analysis (Single strand conformation polymorphism analysis of polymerase chain reaction products).Mutation rates were respectively 22.5%(9/40) and 2.5%(1/40).H-ras mutation was 14.7% (5/34) in 34 HPV-positive，biopsy tissues·The results show:HPV16 E7 transformed gene is a critical gene in the develepment of cervical carcinoma. Mutation of H-ras gene is infrequent in cervical carcinoma. But it is possible to play an important roly in latter stages p⁵³ mutation is infrequent in cervial carcinomal with HPV infection.