| 抗精脒单克隆抗体的制备及其特性鉴定 |
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| 引用本文： | 王德斌,陈智周,范振符,曾明华,田京燕.抗精脒单克隆抗体的制备及其特性鉴定[J].细胞与分子免疫学杂志,1994(1). |
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| 作者姓名： | 王德斌 陈智周 范振符 曾明华 田京燕 |
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| 作者单位： | 中国医学科学院肿瘤研究所 |
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| 基金项目： | 国家“七、五”攻关科研基金 |
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| 摘 要： | 本文用鼠×鼠细胞杂交瘤技术首次建立了抗多胺（精脒）单克隆抗体的杂交瘤细胞系。以此杂交瘤经体外组织培养扩大，并注入同系小鼠腹腔，产生的腹水中抗体滴度达１：１０６。单抗的类别是ＩｇＧ１。单价抗体是由分子量５２ｋＤ和２７ｋＤ组成的复合体。这个单克隆抗体可用于癌症的快速诊断和预后随访。
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| 关 键 词： | 多胺（精脒） 抗体，单克隆 酶联免疫测定 |
Preparation and Characterization of Monoclonal Antibodies against Spermidine |
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| Wang Debin,Chen Zhizhou,Fan Zhenfu,Cao Minghua,Tian Jingyan.Preparation and Characterization of Monoclonal Antibodies against Spermidine[J].Journal of Cellular and Molecular Immunology,1994(1). |
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| Authors: | Wang Debin Chen Zhizhou Fan Zhenfu Cao Minghua Tian Jingyan |
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| Abstract: | A monoclonal antibody Was first prepared after fusion of mouse myeloma cells(Sp2/0-Ag-14)with spleen cells isolated from BALB/c mice immunized with spermidine-bovine serum albhmin conjugate(SPD-BSA).The hybridoma cell line producing antibody specific for spermidihe was grown in tissue culture and then ihjected into the mouse as an ascites tumor giving ascitic fluid with high titres(1:106)measured by ELISA. This monoclonal antibody is IgG1 and a bimolecular comPlex with molecular weight of 52kD and 27kD. The monoclonal antibody may proye to be useful in the rapid diagnosis and evaluation of patients with cancer. |
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| Keywords: | Polyamine(spermidine) antibody monoclonal ELISA |
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