复方丹参片和复方丹参颗粒中三七薄层色谱鉴别的研究

目的:对《中国药典》2010年版一部中复方丹参片和复方丹参颗粒的三七薄层色谱鉴别方法进行了提高,增加了人参皂苷Re对照。方法:采用硅胶G高效预制薄层板,以二氯甲烷-无水乙醇-水(70∶45∶6.5)为展开剂,在相对湿度小于18%的环境下展开。结果:考察了不同的提取方法、展开剂和展开条件,最终确定该方法。对19批复方丹参片和3批复方丹参颗粒进行了试验,人参皂苷Rb1,人参皂苷Rg1,人参皂苷Re和三七皂苷R1均斑点清晰,分离较好。结论:提高后的方法快速、简便,专属性更好,能有效地控制该类制剂的质量。

Study on Identification of Notoginseng (Sanqi) in Compound Danshen Tablets and Compound Danshen Granules by TLC

Objective： To improve the TLC identification of Notoginseng （Sanqi） in Compound Danshen Tablets and Compound Dan- shen Granules in Edition 2010 of Chinese Pharmacopoeia （ Volume I ）. Add ginsenoside Re as a reference standard to the method. Methods ： Precoated HPTLC silica gel 60 plate was used. Developing solvent was dichloromethane-absolute ethanol-water （70：45 ： 6. 5 ） and developed beneath relative humidity 18%. Results： Confirmed the method after studying different extraction methods, developing solvent and developing conditions. The method was applied to 22 samples and the spots of ginsenoside Rb1 , ginsenoside Rg1, ginsenoside Re and notoginsenoside R1 were clear and separated well. The separating degree of ginsenoside Re and notoginsenoside R1 was good. Conclusion ： The improved method is rapid, simple, repeatable. This method can be used for the quality control of this type of agents.