Functional organization of the direct and indirect projection via the reticularis thalami nuclear complex from the motor cortex to the thalamic nucleus ventralis lateralis

Summary The projection systems which arise from the motor cortex to reach the nucleus ventralis lateralis (VL) were investigated in the rat. They included a direct as well as an indirect projection via the reticularis thalami nuclear complex (RT). The investigation was performed in two steps: i) the former concerned the projection to the VL as well as to the RT from individual cortical foci electrophysiologically identified by the motor effects evoked by electrical stimulation; the second step concerned the projection from the RT to functionally defined regions of the VL. The direct projection from the motor cortex to the VL is somatotopically arranged. The projection reciprocates the fiber system directed from the VL to the motor cortex. Thus cortical zones controlling the motor activity of the proximal segments of the limbs project onto the regions of the VL that project back to these same cortical areas. With regard to cortical zones controlling the motor activity of the distal segments of the limbs, they not only project to the region of the VL specifically related to them, but also to the region of the VL associated with the cortical areas responsible for movements of the proximal parts of the same limb. In that case fiber terminals were more dense in the VL region controlling the proximal segment than in the region controlling the distal segment of the same limb. This organization suggests that proximal adjustments may be automatically provided by the motor activity of the distal segments of the same limb. The motor cortex projects to the rostral region of the RT with a precise topographical organization. In particular, the projection shows a dorsoventral organization in the RT in relation to the caudorostral body representation in the motor cortex. The projection which arises from the rostral region of the RT also reaches the VL with a topographical arrangement. It discloses a rostrocaudal organization in the VL in relation to a dorsoventral displacement in the RT. Comparing the projection from the motor cortex to the RT and that from this nuclear complex to the VL it was shown that the regions of the VL and their receptive cortical areas were associated with the same regions of the RT. It was therefore concluded that the motor cortical projection to the VL relayed by the RT is somatotopically organized. In both direct and relayed pathways the projections from hind- and forelimb motor area are segregated, whereas the head projection overlaps, at least partially, the forelimb terminal field. The cortico-VL and the cortico-RT-VL pathways differ by the higher complexity of the former system. Projections from the cortical zones of proximal and distal segments of the limbs largely overlap in RT whereas direct cortico-VL connections disclose a precise complex arrangement. Finally, the possible influence of the two pathways upon thalamic motor relay cells is suggested.Abbreviations: AD nucleus anterodorsalis - AV nucleus anteroventralis - CL nucleus centralis lateralis - LD nucleus laterodorsalis - LGd dorsal lateral geniculate body - LP nucleus lateralis posterior - Po posterior group - RT reticularis thalami nuclear complex - VB ventrobasal complex - VL nucleus ventralis lateralis - VM nucleus ventralis medialis - WGA-HRP wheat germ agglutinin-horseradish peroxidase conjugated