强直性脊柱炎湿热痹阻证血清差异蛋白组学研究

目的?应用蛋白质组学双向电泳和质谱技术，开展强直性脊柱炎湿热痹阻证证候学研究。方法?对强直性脊柱炎湿热痹阻证、寒湿痹阻证、正常对照共3组各30例血清，分别进行双向凝胶电泳，软件分析获取湿热痹阻证组和其他2组的差异蛋白质点，二级质谱鉴定获得差异蛋白。应用Western blot验证差异蛋白。结果?获得7个湿热痹阻证的共同差异蛋白点，经质谱鉴定和SwissProt数据库检索，获得Fibulin-1、二肽基肽酶1、激肽原1、白细胞介素17F突变型、甘露糖结合蛋白C等5个强直性脊柱炎湿热痹阻证差异蛋白，涉及胞外基质蛋白、蛋白酶类、凝血因子、细胞因子、转运蛋白等。Western blot验证甘露糖结合蛋白C的表达结果与蛋白组学检测结果一致。结论?这些蛋白可能涉及强直性脊柱炎湿热痹阻证的发病和病理过程，为进一步研究湿热痹阻证实质提供了依据。 

Differential Serum Proteomic Analysis of Ankylosing Spondylitis Patients with Damp-heat Arthralgia Spasm Syndrome

OBJECTIVE?Proteomic technique was applied to analyze ankylosing spondylitis (AS) patients with damp-heat arthralgia spasm syndrome. METHODS?Serum proteins of AS patients with damp-heat arthralgia spasm syndrome group (n=30) was compared with that of normal control group (n=30) and AS patients with cold-dampness arthralgia spasm syndrome group (n=30) respectively by two-dimensional gel electrophoresis; the common differential protein spots of damp-heat arthralgia spasm syndrome were analyzed by the second order MS. The validation of the differential protein was performed using Western blot. RESULTS?Seven kinds of common differential protein spots were selected and five kinds of proteins were identified by mass spectrometry. They were fibulin 1， dipeptidy l， peptidase 1， kininogen 1， mutant IL 17F， mannose-binding protein C and related to extracellular matrix protein， protease， coagulation factor， cytokine， transport protein， and so on. Results of Western blot were consistent with the proteomic results. CONCLUSION?These proteins are implicated in the pathogenesis and pathological processes of AS with damp-heat arthralgia spasm syndrome， and provide a basis for further studies of the essence of the syndrome in traditional Chinese medicine.