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地骨皮活性成分对高糖致肾小球系膜细胞增殖及细胞外基质的影响
作者姓名:周盈  皮文霞  蔡宝昌  赵文望  黄浦俊
作者单位:南京中医药大学药学院,江苏 南京?210023
摘    要:目的?观察地骨皮中有效成分对高糖刺激的肾小球系膜细胞的增殖以及细胞外基质分泌的影响,研究其对糖尿病肾病的保护机制。方法?体外培养肾小球系膜细胞,分为高糖模型组(葡萄糖浓度为30?mmol/L),空白对照组(葡萄糖浓度为5.5?mmol/L),甜菜碱、山奈酚5个浓度剂量组(0.01、0.1、1、10、100?μmol/L),用MTT法观察系膜细胞增殖情况,ELISA法观察细胞外基质分泌情况。结果?与高糖模型组比较,不同剂量甜菜碱组和山奈酚组,对高糖致肾小球系膜细胞增殖有不同程度的抑制。甜菜碱、山奈酚均能抑制细胞外基质的分泌,与高糖模型组比较有显著性差异(P<0.05~0.01)。结论?地骨皮中有效成分甜菜碱和山奈酚均具有抑制高糖刺激下系膜细胞增殖以及细胞外基质分泌的作用,推测地骨皮中有效成分具有治疗糖尿病肾病的作用。 

关 键 词:甜菜碱    山奈酚    肾小球系膜细胞    糖尿病肾病    地骨皮
收稿时间:3/3/2015 12:00:00 AM
修稿时间:2015/6/24 0:00:00

Effect of Active Components from Lycii Cortex on Proliferation of High Glucose-induced Glomerular Mesangial Cells and Extracellular Matrix
Authors:ZHOU Ying  PI Wen-xi  CAI Bao-chang  ZHAO Wen-wang  HUANG Pu-jun
Abstract:OBJECTIVE?To observe the effect of active components from Lycii cortex on the proliferation of high glucose-induced glomerular mesangial cells (GMCs) and the secretion of extracellular matrix (ECM). To investigate the protective mechanism of diabetic nephropathy. METHODS?GMCs were cultured and divided into high glucose group (final glucose concentration of 30?mmol/L), blank group (final glucose concentration of 5.5?mmol/L), betaine and kaempferol groups of 5 different concentrations of 0.01, 0.1, 1, 10, 100?μmol/L. The proliferation of high glucose-induced GMCs were observed by MTT method and secretion of ECM was detected by ELISA assay. RESULTS?Different doses of betaine and kaempferol inhibited the proliferation of GMCs, and decreased the content of ECM, compared with high glucose group (P<0.05~0.01). CONCLUSION?Betaine and kaempferol can protect the high glucose-induced GMCs through inhibiting the proliferation of GMCs and decreasing the content of ECM. 
Keywords:betaine  kaempferol  glomerular mesangial cells  diabetic nephropathy  Lycii cortex
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