Inhibition of 1,2-Dimethylhydrazine-induced Oxidative DNA Damage by Green Tea Extract in Rat |
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| Authors: | Masao Inagake Tetsuro Yamane Yoshitaka Kitao Kazuhiko Oya Hirohiko Matsuraoto Norikazu Kikuoka Hirohisa Nakatani Toshio Takahashi Hiroshi Nishimura Akio Iwashiraa |
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| Affiliation: | First Department of Surgery, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602;Department of Biochemistry, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602 |
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| Abstract: | Following subcutaneous injection of 1,2-dimethylhydrazine (DMH), which is carcinogenic to rat colon and liver, to Sprague-Dawley rats, a significant increase of 8-hydroxydeoxyguanosine (8-OHdG) was observed in the DNA of colonic mucosa and liver. The 8-OHdG formation reached the maximal level at about 24 h after the DMH injection. On the other hand, no increase of 8-OHdG was observed in the DNA of the kidney. Drinking green tea extract (GTE) for ten days prior to the DMH injection significantly inhibited the formation of 8-OHdG in the colon. These findings demonstrate that DMH causes oxidative damage to the DNA of its target organ, and that GTE protects colonic mucosa from this oxidative damage. |
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| Keywords: | 8-Hydroxydeoxyguanosine Green tea 1,2-Dimethylhydrazineon cancer |
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