The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing |
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Authors: | Shen Haihong Zheng Xuexiu Luecke Stephan Green Michael R |
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Affiliation: | Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea. haihongshen@gist.ac.kr |
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Abstract: | The U2AF35-related protein Urp has been implicated previously in splicing of the major class of U2-type introns. Here we show that Urp is also required for splicing of the minor class of U12-type introns. Urp is recruited in an ATP-dependent fashion to the U12-type intron 3' splice site, where it promotes formation of spliceosomal complexes. Remarkably, Urp also contacts the 3' splice site of a U2-type intron, but in this case is specifically required for the second step of splicing. Thus, through recognition of a common splicing element, Urp facilitates distinct steps of U2- and U12-type intron splicing. |
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Keywords: | Urp U2AF35 splicing spliceosome assembly U2-type intron U12-type intron |
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