硒、维生素A、E对人类乳腺癌细胞增殖的影响

本文研究了Se、VA和VE及其联合作用对人类乳腺癌细胞BCaP-37增殖的影响,结果提示Se对该细胞的增殖有双向作用,浓度高于5μM抑制细胞生长,低于该浓度则刺激细胞生长。醋酸维生素A酯浓度在1.0—10.0mg/L范围内对该细胞生长有明显的抑制。维生素A酸对该细胞的生长仅有很弱的抑制作用。VE对细胞也有弱抑制生长的作用。在稍高于生理浓度的水平下,Se(5μm)与VA(2mg/L)和VE(20mg/L)三者联合处理细胞,细胞核酸含量明显降低,提示Se、VA和VE能协同抑制BCaP-37细胞的增殖,而且这种抑制有可逆的趋势。联合使用Se和VA或VE未观察到二者间对细胞生长有协同抑制。上述结果表明联合使用Se、VA和VE对乳腺癌的防治可能是有益的。

EFFECTS OF SELENIUM, VITAMIN A AND E ON HUMAN BREAST CANCER CELL PROLIFERATION

The study was conducted in vitro with human breast cancer cells BCaP-37, to determine the effects of selenium, vitamin A, vitamin E and a combination of these three nutrients on cell proliferation and cellular nucleic acid content. Selenium as sodium selenite had two phases of effect on cancer cell proliferation: the low concentrations of selenium (less than 5 μM) stimulated cell growth and increased the cellular nucleic acid content; the high concentrations (more than 5 μM) depressed cell growth and reduced the cellular nucleic acid content with dose-dependence. Vitamin A acetate inhibited cancer cell growth significantly, but vitamin A acid inhibited to some extent, and was less effective than vitamin A acetate. Vitamin E had less inhibitory effect compared to vitamin A acetate and the inhibitory percentages were lower than 40% in all treatment groups. Combination of selenium (5 μM) and vitamin E (20mg/L) or selenium and vitamin A acetate (2mg/L), no synergism for the reduction of the contents of cellular nucleic acids (DNA and RNA) were observed. The combination of selenium, vitamin A acetate and vitamin E at such levels reduced cellular DNA and RNA contents obviously; RNA content was significantly lower than any other treatment group and was reduced synergis-tically. It was indicated that the combination of selenium, vitamin A acetate, vitamin E was synergistic for inhibition of cell proliferation. Results also showed the reversible tendency in the inhibition of cell proliferation by combination of these three nutrients. It was suggested that combination of selenium, vitamin A and E might be benificial for the prevention and adjuvant treatment of human breast cancer.