抑郁模型大鼠结肠组织中SOD、MDA和COX-2的异常

目的观察抑郁症导致的大鼠结肠组织中超氧化物歧化酶(SOD)、丙二醛(MDA)和环氧合酶-2(COX-2)的异常.方法建立wistar大鼠的慢性应激抑郁模型.实验动物随机分为:正常组和模型组,每组各10只.采用紫外分光光度计检测大鼠结肠组织中SOD和MDA的含量;采用免疫组化方法检测结肠组织COX-2的表达.结果 与正常组比较,抑郁模型大鼠结肠组织中MDA的含量显著升高, COX-2的表达显著上调(P <0.01);而SOD含量显著降低(P <0.01).结论 抑郁大鼠结肠的损害可能与其结肠组织中氧自由基与花生四烯酸代谢异常有关.

Abnormity of SOD, MDA and COX-2 in colon of depression rats

Objective To investigate the abnormity of superoxide dismutase (SOD), malondialdehyde (MDA) and cyclooxygenase-2 (COX-2) in colon of rats caused by depression.Methods The chronic stress-induced model of depression of wistar rats was produced . The experimental animals were randomly divided into model control and normal control in the study. The levels of SOD and MDA were assayed with ultraviolet spectrophotometry. The expression of COX-2 in colonic tissue was detected with immunohistochemistry.Results Enhanced colon inflammatory response and oxidative stress were observed in the chronic stress-induced depression model of rats, which manifested as the significant increase of MDA, as well as the expressions of COX-2 in the colon tissue, while the colonic SOD activity was significantly decreased compared with the normal control (MDA: 10.34±2.77 vs 2.55±0.70; COX-2: 53.26±8.16 vs 4.87±1.05; SOD:53.39±11.15 vs 84.45±22.31; P <0.01). Conclusion Depression can induce injuries on colon of rats, which was probably due to the abnormity of metabolism of oxygen free radical (OFR) and arachidonic acid.