胶质瘤端粒酶活性与瘤增殖能力的关系研究

目的：通过对胶质瘤端粒酶活性的测定和Ki-67核抗原免疫组化染色，了解端粒酶活性与胶质瘤预后的增殖潜能之间的关系。方法：对42例胶质瘤组织标本，用端粒酶PCR ELISA盒进行端粒酶活性半定量一，免疫组织化学染色检查Ki-67抗原。比较各组肿瘤端粒酶活性，Ki-67表达的差异，结果：正常人脑血组织无端粒酶活性。胶质细胞瘤Ⅱ级组1／15例（6．7％），Ⅲ级组5／13（38．5％）、Ⅳ级组10／14例（71．4％）端粒酶阳性，各组之间阳性率有显著差异，Ki-67阳性细胞表达指数则随肿瘤恶性程度增高而增加结论：端粒酶在胶质瘤的发展中起重要作用，它是脑肿瘤发展过程中，晚期细胞分裂旺盛的分子标志。

Study of Telomerase Activity and Proliferation of Brain Glioma

Through detecting the telomerase activity of brain gliomas and combing with ki-67 immunohistochemical staining analysis, we are trying to reveal the relationships between the telomerase activity and the tumorigenesis, development, and proliferation of gliomas. Methods: Telomerase activity in glioma tissues from 42 patients with glioma were detected by telomerase PCR and ELISA. Ki-67 antigen of the specimen was examined by immunochemical stains. Results: Normal adult brain tissues had no detectable telomeras activity. Whereas, 1/15, 5/13, and 10/14 cases of gliomas grade II, III, IV had detectable telomerase activity (6.7% , 38.5% and 71.4% respectively). The positive rate of telomeras activity was significant higher in high-grade glioma. Conclusion: Telomerase may play an important role in the development of malignant glioma. The presence of telomerase in tumor may be a marker to predicate the worse prognosis.