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尼美舒利对人食管癌细胞Eca-109 COX-2表达及生长的抑制作用
引用本文:刘俊茹,齐凤英,李丽,左连富,郭建文,刘江惠.尼美舒利对人食管癌细胞Eca-109 COX-2表达及生长的抑制作用[J].中国药理学通报,2005,21(9):1084-1088.
作者姓名:刘俊茹  齐凤英  李丽  左连富  郭建文  刘江惠
作者单位:1. 河北医科大学病理学教研室,河北,石家庄,050017
2. 河北医科大学肿瘤研究所,河北,石家庄,050011
摘    要:目的研究环氧化酶-2(COX-2)选择性抑制剂尼美舒利对人食管癌Eca-109细胞株COX-2表达和细胞增殖及凋亡的影响。方法MTT法测定尼美舒利对人食管癌Eca-109细胞增殖的抑制率;RT-PCR法检测Eca-109细胞COX-2mRNA表达变化;流式细胞仪检测COX-2蛋白表达、细胞周期时相分布及凋亡率的变化;光镜和琼脂糖电泳法进一步观察细胞凋亡。结果尼美舒利对人食管癌Eca-109细胞有较强的抑制作用,有明显的时间和浓度依赖性;呈浓度依赖性下调Eca-109细胞COX-2 mRNA及蛋白表达;可使Eca-109细胞G0/G1期比例增高,S期细胞减少,增殖指数降低,凋亡细胞增多。结论尼美舒利可能通过对COX-2表达的下调而诱导凋亡和细胞周期阻滞,从而抑制人食管癌Eca-109细胞生长。

关 键 词:尼美舒利  环氧化酶-2  人食管癌Eca-109细胞株  凋亡
文章编号:1001-1978(2005)09-1084-05
修稿时间:2005年1月4日

Inhibitive effects of nimesulide on cyclooxygenase-2 expression and proliferation in human esophageal carcinoma Eca-109 cells
LIU Jun-Ru,QI Feng-ying,LI Li,ZUO Lian-fu,GUO Jian-wen,LIU Jiang-hui.Inhibitive effects of nimesulide on cyclooxygenase-2 expression and proliferation in human esophageal carcinoma Eca-109 cells[J].Chinese Pharmacological Bulletin,2005,21(9):1084-1088.
Authors:LIU Jun-Ru  QI Feng-ying  LI Li  ZUO Lian-fu  GUO Jian-wen  LIU Jiang-hui
Abstract:Aim To study the effects of selective cyclooxygenase-2 (COX-2) inhibitor,nimesulide,on COX-2 expression, cell proliferation and apoptosis of human esophageal carcinoma Eca-109 cell lines. Methods MTT assay was used to observe the proliferative effect;COX-2 mRNA expression was evaluated with RT-PCR; COX-2 protein expression,cell cycle and apoptosis were analyzed with flow cytometry;microscope and agarose gel electrophoresis of DNA were also used to observe the apoptosis. Results Nimesulide significantly inhibited the proliferation of Eca-109 cell lines in a time and dose-depenent fashion, down-regulated the expression of COX-2 mRNA and COX-2 protein in a dose-dependent fashion;nimesulide also decreased the proliferation index and the proportion of cells in S phase, meanwhile increased the proportion of cells in G_0/G_1 phase and induced apoptosis. Conclusion COX-2 selective inhibitor nimesulide inhibits proliferation,induces apoptosis and cell cycle arrest of human esophageal cells via down-regulation of COX-2 expression.
Keywords:nimesulide  Cyclooxygenase-2  human esophageal carcinoma Eca-109 cell line  apoptosis
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