Syntheses and Immunologic Properties of Escherichia coli O157 O-Specific Polysaccharide and Shiga Toxin 1 B Subunit Conjugates in Mice |
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| Authors: | Edward Konadu Arthur Donohue-Rolfe Stephen B Calderwood Vince Pozsgay Joseph Shiloach John B Robbins and Shousun C Szu |
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| Institution: | National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2720, USA. |
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| Abstract: | Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers, conjugate vaccines of detoxified lipopolysaccharide (LPS) elicited bactericidal antibodies to E. coli O157. Here, the detoxified LPS was conjugated with improved schemes to the nontoxic B subunit of Stx1. Mice injected with these bivalent conjugates elicited both bactericidal antibodies to E. coli O157 and neutralization antibodies to Stx1. |
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