慢性乙型肝炎病人单个核细胞γ干扰素的诱生和T淋巴细胞亚群的研究

本文对28例慢乙肝病人的外周血单个核细胞进行ConA诱生γ干扰素的测定,同时用T细胞单克隆抗体系统检测其T细胞亚群。结果发现,慢乙肝病人ConA诱生的γ干扰素效价为7.858±1.483log2U/ml,而正常对照组为7.839±0.621log2U/ml,两组间无显著性差异,但在慢乙肝病人组内个体间IFN-γ的效价差别显著大于对照组(P<0.05).慢乙肝病人组T细胞亚群的T_4/T_3比例与对照组无显著差异。但IFN-γ反应低下组的5人均有T_4/T_3比例异常。在28例病人中有14例T_4/T_3比例异常(高于或低于正常),且20例HBeAg阳性病人中有12例T_4/T_3比例异常;8例HBeAg阴性病人中仅2例T_4/T_3比例异常。

INDUCTION OF γ-INTERFERON AND T LYMPHOCYTE SUBSETS IN CHRONIC HEPATITIS B PATIENTS.

This study deals with the detection of IFN-γ induced by Con A from peripheral mononuclear cells in 28 cases of chronic hepatitis B patients and 20 normal controls for comparison and the identification of T subsets as defined by monocloned antibodies. The mean titer of IFN-γ induced by Con A in chronic hepatitis B patients was 7.858 ± 1.483 log 2U/ml, the mean titer of normal controls was log 2U/ml, there wad no significant difference between these two groups(P>0.05).however, the Induction of, IFN-γ in the patients group showed a wide range of variation among different individuals, with the coefficient of variability (C. V.) of 18.9%, it was greater than that of control group with C.V. of 7.9% only. The T4/T8 ratio of chronic hepatitis B patients was not significantly different with normal controls (p>0.05). But in patients group, the T4/T8 ratio also sh owed a wide range of variation. Among 28 chronic hepatitis B patients, the T4/T8 ratio were abnormal in 14 patients(8 decreased, 6 increased), it was more prominent in HBeA-g positive patients. In 5 patients with decreased production of IFNγ, the T4/T8 ratio was all abnormal(3 decreased, 2 increased). It suggested that in patients which IFN γ production was decreased might have greater derangement in cellular immunity.