骨形态形成蛋白用于大面积关节软骨缺损修复的实验研究

目的：将骨形态形成蛋白用于大面积关节软骨缺损的修复，探讨应用方法，观察修复效果。方法：在51只成年兔股骨的髌髁关节面上制造5mm×10mm的骨软骨缺损，深3～5mm。缺损内分别填充骨形态形成蛋白和纤维蛋白粘合剂复合物（BMP／FS）、BMP、FS，植入物均用自体游离骨膜覆盖。术后2、4、8、12周对缺损修复情况行大体和组织学观察。结果：BMP／FS组，8周时软骨下骨再生已完成，12周表层新生软骨组织结构接近正常，修复效果明显优于其它组。结论：骨膜覆盖固定BMP／FS，是修复大面积关节软骨缺损的较好方法。

Repair of large osteochondral defect with the composite of bone morphogenetic protein(BMP) and fibrin sealant, an experimental study in the rabbit

Objective: The purpose of this study was to seek a new method for repair of large osteochondral defect with bonemorphogenetic protein(BMP). Methods: a large osteochondral defect, 5 by 10mm in size, 3 to 5mm in depth, was created inthe patellar groove of each knee of 51 adult New Zealand rabbits.The composite of BMP and fibrin sealant (BMP/FS)wasimplanted into the defect with BMP alone and FS alone as controls. The implanted substance was covered with free autologous tibial periosteum. The defects covetal with periosteum alone or without any implantation also served as controls. Thehealing of the defects were assessed by gross and micoscopic examination at 2,4, 8and 12 weeks. Results: The defectstreated with BMP/FS composite and periosteum implantation showed that subchondral bone regenerated well at 8weeks, andnew cartilage tended to give normal appearance at 12 weeks, much better than those of the defects treated with other means.Conclusions:The BMP/FS composite covetal with periosteum could achieve early repair of large osteochondral defect.