| HPLC法测定布渣叶中山柰素的含量 |
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| 引用本文： | 曾聪彦,李依信,梅全喜,戴卫波,钟希文. HPLC法测定布渣叶中山柰素的含量[J]. 中医药学报, 2010, 38(5): 87-89 |
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| 作者姓名： | 曾聪彦 李依信 梅全喜 戴卫波 钟希文 |
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| 作者单位： | 1. 广州中医药大学附属中山中医院,广东,中山,528400
2. 广东省云浮市药品检验所,广东,云浮,527300 |
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| 基金项目： | 中山市科技计划资助项目 |
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| 摘 要： | 目的：建立布渣叶中山柰素的含量测定方法,为其制定质量标准提供依据。方法：采用高效液相色谱法对布渣叶药材山柰素进行含量测定。色谱柱：Diamonsil（钻石）C18（250×4.6mm,5μm）;流动相：甲醇-0.4%磷酸溶液（52∶48）;流速：1.0mL/min;柱温：30℃;检测波长：367nm。结果：山柰素进样量在0.01021ug~0.20420ug范围内与峰面积呈良好的线性关系（r=0.9999）。平均加样回收率为99.13%,RSD为1.05%（n=6）。结论：所建立的方法简便、快捷,重现性好,可用于该药材的质量评价。
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| 关 键 词： | 布渣叶 山柰素 高效液相色谱法 |
Determination of Kaempferol in Microcos Paniculata by HPLC |
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| ZENG Cong-yan,LI Yi-xin,MEI Quan-xi,DAI Wei-bo,ZHONG Xi-wen. Determination of Kaempferol in Microcos Paniculata by HPLC[J]. Acta Chinese Medicine and Pharmacology, 2010, 38(5): 87-89 |
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| Authors: | ZENG Cong-yan LI Yi-xin MEI Quan-xi DAI Wei-bo ZHONG Xi-wen |
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| Affiliation: | 1.Zhongshan Hospital,Guangzhou University of Chinese Medicial,Zhongshan 528400,China;2.Yunfu Drug Control Institute,Yunfu 527300,China) |
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| Abstract: | Objective：To establish the quantitative methods of kaempferol in Microcos paniculata and control its quanlity.Methods：The content of kaempferol in Microcos paniculata was determined by HPLC.The chromatgraphic conditions were as follows：Diamonsil C18（250×4.6mm,5μ）,mobile phrase was methanol-0.4% phosphoric acid（52∶48）,flow rate being 1.0ml/min,column temperature at 30℃,detection wavelength being 367nm.Results： Kaempferol showed a good linear relationship in the range of 0.01021ug~0.2042ug（r=0.9999）,the average recovery was 99.13% and RSD was 1.05%.Conclusion：The established methods are simple and rapid with good reproducibility,and can be used for the quality control of Microcos paniculata. |
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| Keywords: | Microcos paniculata Kaempferol HPLC |
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