血管紧张素转换酶和血管紧张素受体基因多态性与冠心病的关系

目的　探讨深圳地区冠心病 (CAD)与血管紧张素转换酶 (ACE)基因与血管紧张素 的 1型受体 (AT1R)基因多态性的关系。方法　分别采用 PCR及 PCR- Afl II酶切法 ,检测 89例 CAD患者和 14 8例健康对照的 ACE和AT1R基因型。结果　 CAD组与对照组比较 ,ACE DD基因型频率 (2 4 .7%比 8.1% ,P<0 .0 1)及 D型等位基因频率 (4 4 .4 %比 33.4 % ,P<0 .0 5 )均为升高。 CAD组与对照组 AT1R基因型频率分布无显著性差异 (P>0 .0 5 )。携带 AT1R C等位基因的个体患 CAD的风险与其同时携带 ACE DD基因型无关 (P>0 .0 5 )。结论　深圳地区CAD的发生和发展可能与 ACE基因 I/ D多态性有关 ,而与 AT1R基因 A116 6 C多态性无关

ACE and AT1R gene polymorphisms and coronary artery disease

Objective To investigate the relationship betweer polymorphism of angiotensin converting enzyme (ACE) gene,angiotensin type 1 receptor(AT1 R) gene and Coronary artery disease(CAD) Methods ACE genotypes and ATlR genotypes were determined by PCR and PCR-based restriction enzyme analysis method in 89 patients with CAD and 148 normal controls. Results The frequencies of DD genotype and D alleles of ACE I/D polymorphism showed significant difference between CAD and controls(DD genotype was 24.7% vs 8.1%, P<0.01; allele D was 44.4% vs 33.4%,P<0.05) ; there is no difference AIIR gene potymorphigmg in the distribution of the AT1R genotypes between two groups(P>0.05).There was no significant synergistic effects of ACE and ATIR gene polymorphisms on the risk of CAD. Conclusion The occurrence and development of CAD may be associated with ACE I/D gene polymorphism but not with AT1R A1166C polymorphism.