弓形虫病诊断试剂盒阴性血清保存方法的研究

目的：研究SPA-ELISA弓形虫病诊断试剂盒不同稀释液及灭活处理对阴性血清的保存效果。方法：正常人阴性血清（n=5）分别用0．01MPBS缓冲液、1％胎牛血清、15％甘油1：100进行稀释后保存于4℃，分别于稀释后1d、1周、2周、4周、2个月、3个月用SPA-ELISA检测阴性血清OD410比较其变化情况：同时观察灭活对阴性血清的影响。结果：用15％甘油稀释的阴性血清可以保存至3个月，用0．01M PBS缓冲液和1％胎牛血清稀释的阴性对照一个月之后保存效果较差。结论：15％甘油稀释的阴性血清可以比较长期稳定的保存．保证了SPA-ELISA弓形虫病诊断试剂盒的稳定性。

The study on methods of storing the negative serum of diagnostic kit of toxoplasmosis

Objective;To investigate the storing effects of negative serum of diagnostic kit of Toxoplasma gondii,after diluted with different solutions and inactivation.Methods;Negative human serum samples (n=5) diluted 1;100 with 0.01 M PBS buffer,1% BSA and 15% glycerol respectively were tested by SPA-ELISA at 1 day,1 week,2 weeks,4 weeks,2 months,3 months.The changes and difference of OD410 of them were compared.The effects of inactivation of serum were compared with that with no inactivation.Results;It showed that negative serums diluted with 15% glycerol can be well stored until 3 months.Comparatively,Negative serums kept unstable in 0.01M PBS buffer and 1% BSA after 1 month.Conclusion;Negative serum can be stably stored in 15% glycerol in long term,which ensures the stability of SPA-ELISA diagnostic kit of Toxoplasmosis.