海带胞壁多糖抑制血栓形成和血液凝固的实验研究

目的研究海带胞壁多糖对血栓形成和血液凝固的影响.方法大鼠连续腹腔注射海带胞壁多糖或生理盐水3d后,通过动-静脉旁路血栓形成、电刺激颈总动脉血栓形成和结扎下腔静脉血栓形成的实验方法,分别比较生理盐水组与海带胞壁多糖高剂量组(100mg/kg)和低剂量组(20mg/kg)血栓重量和血栓堵塞时间;测定颈总动脉血栓形成后的大鼠全血凝固时间(CT)、血浆凝血酶原时间(PT)和活化部分凝血活酶时间(APTT),观察海带胞壁多糖对血液凝固的影响.结果与生理盐水组比较,海带胞壁多糖高剂量组和低剂量组均能降低动脉血栓和静脉血栓湿重,延长电刺激颈总动脉血栓堵塞时间及CT和APTT,高剂量组还能延长PT,具有统计学意义;低剂量组虽显示出延长PT的作用,但无统计学意义.结论海带胞壁多糖具有抑制大鼠血栓形成和血液凝固的作用.

Experimental Studies on Cell Wall Polysaccharide of Thallus Laminariae In Inhibiting Thrombosis and Blood Coagulation

Objective To study the effect of cell wall polysaccharide (CWP) of Thallus Laminariae (TL) on thrombosis and blood coagulation.Methods Thrombosis was induced by arteriovenous shunt,ligation of inferior vena cava and electric stimulation of common carotid artery,after intraperitoneal injection of CWP or nomal saline for 3 days.The weight of thrombus and the occlusion time were examined in the normal saline group and high-dose (100 mg/kg) and low-dose (20 mg/kg) groups of CWP of TL.Blood coagulation time (CT),plasma prothrombin time (PT) and partial thromboplastin time (APTT) were measured to observe the effect of CWP on blood coagulation.Results Compared with the control group,both high-dose CWP of TL and low-dose CWP can obviously decrease the weight of thrombus and prolong the occlusion time,CT and APTT.High-dose CWP could also prolong PT obviously,the differences being significant.Low-dosa CWP also prolong PT,but the difference was not significant statistically.Conclusion CWP of TL can inhibit the thrombus formation and blood coagulation in rats.