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烫伤后脂多糖受体CD14和肿瘤坏死因子-α基因表达的变化规律
引用本文:Fang W,Yao Y,Shi Z,Yu Y,Wu Y,Lu L,Chang G,Sheng Z. 烫伤后脂多糖受体CD14和肿瘤坏死因子-α基因表达的变化规律[J]. 中华外科杂志, 1999, 37(5): 271-273
作者姓名:Fang W  Yao Y  Shi Z  Yu Y  Wu Y  Lu L  Chang G  Sheng Z
作者单位:解放军第三○四医院创伤外科中心,解放军第二○八医院外四科
基金项目:军队杰出中青年人才专项基金,全军“九五”医药卫生科研基金,中国工程科技奖助基金
摘    要:目的 观察烫伤后机体主要脏器脂多糖受体CD14(CD14)和肿瘤坏死因子-α(TNF-α)mRNA的表达的变化规律及二者的相互关系。方法 采用大鼠35%Ⅲ度烫伤模型,分别于伤前,伤后12,24,48,72小时活杀动物,留取组织标本或分离腹腔巨噬细胞,检测组织或巨噬细胞CD14,TNF-αmRNA表达。结果 烫伤后肝,肺,肾,肠等组织CD14mRNA水平均有不同程度增高,分别于伤后12小时和48小时

关 键 词:肿瘤坏死因子α CD14 脂多糖受体 烧伤

Gene expression of lipopolysaccharide receptor CD14 and tumor necrosis factor-alpha in rats after thermal injury
Fang W,Yao Y,Shi Z,Yu Y,Wu Y,Lu L,Chang G,Sheng Z. Gene expression of lipopolysaccharide receptor CD14 and tumor necrosis factor-alpha in rats after thermal injury[J]. Chinese Journal of Surgery, 1999, 37(5): 271-273
Authors:Fang W  Yao Y  Shi Z  Yu Y  Wu Y  Lu L  Chang G  Sheng Z
Affiliation:Trauma Research Center, 304th Hospital, People's Liberation Army, Beijing 100037.
Abstract:Objective To investigate changes in lipopolysaccharide receptor CD14 and tumor necrosis factor (TNF) gene expression in vital organs, and their significance in the pathogenesis of systemic inflammation after burns. Methods Wistar rats were subjected to a 35 percent fullthickness scald injury, then sacrificed preburn, and postburn 12, 24, 48, 72 hours, respectively. Tissue samples from liver, kidney, lung and intestine were collected to measure CD14 and TNF mRNA expression. Peritoneal macrophages were harvested by peritoneal lavage to determine CD14 mRNA expression. Results CD14 mRNA expression increased markedly after thermal injury, peaking at both 12 and 48 hours. Likewise, CD14 mRNA levels were significantly upregulated in peritoneal macrophages. Gene expression of TNF elevated markedly in liver, lungs, and kidneys after acute insults. Positive correlation was found between CD14 mRNA and TNF mRNA in liver,lung, and kidney tissues. Conclusions5BZThermal injury per se can markedly upregulate both CD14 and TNF gene expression in various organs. Excessive CD14 mRNA expression may enhance synthesis and release of TNF stimulated by endotoxin translocation, and the interaction between CD14 and TNF may play an important role in mediating multiple organ damage secondary to major burns.
Keywords:BurnsEndotoxinsTumor necrosis factorGene expressionCD14  
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