烫伤后脂多糖受体CD14和肿瘤坏死因子-α基因表达的变化规律

目的 观察烫伤后机体主要脏器脂多糖受体ＣＤ１４（ＣＤ１４）和肿瘤坏死因子－α（ＴＮＦ－α）ｍＲＮＡ的表达的变化规律及二者的相互关系。方法 采用大鼠３５％Ⅲ度烫伤模型,分别于伤前,伤后１２,２４,４８,７２小时活杀动物,留取组织标本或分离腹腔巨噬细胞,检测组织或巨噬细胞ＣＤ１４,ＴＮＦ－αｍＲＮＡ表达。结果 烫伤后肝,肺,肾,肠等组织ＣＤ１４ｍＲＮＡ水平均有不同程度增高,分别于伤后１２小时和４８小时

Gene expression of lipopolysaccharide receptor CD14 and tumor necrosis factor-alpha in rats after thermal injury

Objective To investigate changes in lipopolysaccharide receptor CD14 and tumor necrosis factor (TNF) gene expression in vital organs, and their significance in the pathogenesis of systemic inflammation after burns. Methods Wistar rats were subjected to a 35 percent fullthickness scald injury, then sacrificed preburn, and postburn 12, 24, 48, 72 hours, respectively. Tissue samples from liver, kidney, lung and intestine were collected to measure CD14 and TNF mRNA expression. Peritoneal macrophages were harvested by peritoneal lavage to determine CD14 mRNA expression. Results CD14 mRNA expression increased markedly after thermal injury, peaking at both 12 and 48 hours. Likewise, CD14 mRNA levels were significantly upregulated in peritoneal macrophages. Gene expression of TNF elevated markedly in liver, lungs, and kidneys after acute insults. Positive correlation was found between CD14 mRNA and TNF mRNA in liver,lung, and kidney tissues. Conclusions5BZThermal injury per se can markedly upregulate both CD14 and TNF gene expression in various organs. Excessive CD14 mRNA expression may enhance synthesis and release of TNF stimulated by endotoxin translocation, and the interaction between CD14 and TNF may play an important role in mediating multiple organ damage secondary to major burns.