胰岛与Fas配体阳性睾丸细胞共同移植产生免疫豁免

目的通过胰岛与睾丸细胞共同移植诱导胰岛移植物长期生存。方法胶原酶、胰蛋白酶、Ｄｎａｓ酶消化制备睾丸ｓｅｒｔｏｌｉ细胞培养４８小时;纯化的供体（Ｗｉｓｔａｒ大鼠）胰岛５００个培养后的睾丸ｓｅｒｔｏｌｉ细胞共同移植,移植前后不用全身性免疫抑制剂,ＴＵＮＥＬ法标记胰岛移植物周围凋亡的淋巴细胞,ＳＡＢＣ法测定共同移植物ＦａｓＬ稳定表达情况。结果胰岛与１×１０７个睾丸ｓｅｒｔｏｌｉ细胞共同移植可以纠正链脲酶素所至的糖尿病高血糖状态达６０天（有效率达１００％,６／６）,单纯的胰岛移植或与１×１０５个睾丸ｓｅｒｔｏｌｉ细胞共同移植,移植物仅能存活５～６天。移植物周围可见明显的凋亡的淋巴细胞存在。结论胰岛与Ｆａｓ配体阳性睾丸细胞共同移植可以诱导局部免疫豁免,使移植物长时间存活。

Co-transplantation of islet and allogeneic testicular cell induced immune privilege

OBJECTIVE: To induce islet allograft long-term survival through co-transplantation islet with sertoli cell. METHODS: Testicular sertoli cell was prepared by digestion of collagenase, trypsin and Dnase, and cultured for 48 hours. Purified donor (Wistar rat) islet was co-transplanted with allergic sertoli cell, in the absence of systemic immunosuppression. TUNEL (terminal deoxynucleotidyl transferase-mediated X-dUTP nick end labeling) was used to label the apoptosis of lymphocytes surrounding the islet graft. The SABC method was used to label the expression of Fas ligand in co-transplantation. RESULTS: Islet co-transplantation with 1 x 10(7) sertoli cell reversed the diabetic state for more than 60 days in 100% (6/6) of the chemically diabetic SD recipients. Similar grafts consisting of islet alone or islet plus 1 x 10(5)-sertoli cell survived only for 5 - 6 days. Apoptosis of the lymphocytes surrounding the islet was quite clear. CONCLUSIONS: Co-transplantation islet with FasL(+) sertoli cell induces local immune privilege and allows long-term graft survival without systemic immunosuppression.