| 一种新的皮肤活力检测方法的研究 |
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| 引用本文： | Jia X,Yang H,Liss K,McGann LE,Tredget EE. 一种新的皮肤活力检测方法的研究[J]. 中华外科杂志, 1999, 37(3): 183-185 |
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| 作者姓名： | Jia X Yang H Liss K McGann LE Tredget EE |
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| 作者单位： | 解放军第三○四医院烧伤研究所,加拿大阿尔伯塔大学医学院低温医学实验室 |
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| 基金项目： | 军队留学回国人员医学科研启动基金,加拿大国家医学研究理事会基金 |
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| 摘 要： | 探索一种四氮唑盐法对皮肤活力检测及其对储存异体皮质量鉴定的效果。方法以四氮唑盐法对猪皮片和人皮片活进行了实验性检测。对试剂的剂量,反应时间,皮片厚度,皮片面积、皮片重量等实验的选择进行了研究。
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| 关 键 词： | 皮肤移植 烧伤 器官保存 皮肤活力 四氮唑盐法 |
Experimental condition for WST-1 assay of skin viability |
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| Jia X,Yang H,Liss K,McGann L E,Tredget E E. Experimental condition for WST-1 assay of skin viability[J]. Chinese Journal of Surgery, 1999, 37(3): 183-185 |
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| Authors: | Jia X Yang H Liss K McGann L E Tredget E E |
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| Affiliation: | Burn Institute, 304th Hospital, People's Liberation Army, Beijing 100037. |
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| Abstract: | OBJECTIVE: To investigate a new tetrazoliium salt assay (WST-1) of detecting tissue and cells of preserved skin. METHODS: With WST-1, viability of porcine and human skin split were assessed. The experimental condition such as dosage of agents, thickness of skin split, time of reaction, area of skin split and weight of skin split were determined. RESULTS: Optimal experimental condition of skin split should be 10 mm diameter and 0.3 mm thick, incubation for 3 hours at 37 degrees C, and reagent dosage of 20 microl. CONCLUSIONS: As a reagent for detecting cell viability, WST-1 is successfully applied to the assessment of skin viability. |
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