小肠移植排斥反应期移植肠粘膜细胞凋亡的研究

目的 明确移植肠上皮细胞凋亡在小肠移植排斥反应中的意义及诊断价值。方法 选用近交系大鼠Ｆ３４４／Ｎ和Ｗｉｓｔａｒ／Ａ进行全小肠异位移植,实验分４组。第１组：非基本对照 ;第２组;同基因移植组;第３组;异基因移植组;第４组;异基因移植加环孢霉素Ａ治疗组。术的第３、５、７天取移植肠进行病理学检查,采用ＴｄＴ介导的脱氧核苷酸原位末端标记法（ＴＵＮＥＬ）检测移植肠上皮细胞凋亡。结果 病理学检查显示第３组大

Apoptosis of small intestinal epithelial cells in small intestinal allograft rejection

Objective To investigate apoptosis of epithelial cells during small intestinal allograft rejection in rats. Methods Heterotopic small intestinal transplantation was performed with inbred rat F344/N(RT1l)and inbred rat Wistar/A(RT1Ak,RT1Ed). All recipients were divided into four groups:group, nonoperative control (Wistar); group , isograft control (WistarWistar); group , allograft (F344Wistar );group , treatment control e A(6 mgkg-1d-1)]. The grafts were harvested on day 3, 5,7 after operation. All graft samples were subjected to histological examination and apoptotic cells of graft epithelial cells with terminal deoxynucleotidyl transferasemediated dUTP nickend labeling (TUNEL). ResultsT5BZHistologically mild acute rejection occurred on day 3 after operation in group ,moderate acute rejection on day 5 after operation,severe acute rejection on day 7 after operation. But none of group had the histological evidence of acute rejection and the histological evidence of group indicated that Cyclosporine A could effectively controlled small intestinal acute allograft rejection. The TUNEL showed that the number of apoptotic cells per crypt in group was significantlly higher than that of the other three control groups on day 3 and day 5 after operation(P<001). The epithelial mucosa in group completely sloughed on day 7 after operation. Conclusions Apoptosis plays an important role in small intestinal allograft rejection. Detection of apoptotic cells with TUNEL could be a valuable tool for the diagnosis of small intestinal allograft rejection.