胆囊结石病胆囊排空与缩胆囊素受体基因表达关系的研究

目的探讨胆石病患者缩胆囊素（ＣＣＫＡ）受体基因表达及其与胆囊排空功能的关系。方法２０例单纯胆囊结石病患者和１０例健康正常人,术前用Ｂ超测定胆囊排空功能,另以８例因意外死亡无胆囊结石患者胆囊标本为测定ＣＣＫＡ受体基因表达的正常对照。ＣＣＫＡ受体ｍＲＮＡ表达的测定用ＲＴＰＣＲ方法。结果胆石病组胆囊排空功能明显异常。胆石病组ＣＣＫＡ受体ｍＲＮＡ表达量（０６３３±０１６７）明显低于对照组（０９４４±０２３０）（Ｐ＜００１）;在胆石病患者中,收缩减弱组的ｍＲＮＡ表达（０５４４±０１２４）又明显低于收缩正常组（０７６８±０１３１）（Ｐ＜００５）,且ＣＣＫＡ受体ｍＲＮＡ表达的变化与胆囊排空率呈显著正相关（ｒ＝０９２５,Ｐ＜００１）。结论胆石病患者胆囊ＣＣＫＡ受体ｍＲＮＡ表达降低,此降低的基因表达与胆石病胆囊排空功能损害有关。

Correlation between gene expression of CCK-A receptor and gallbladder emptying in gallstone patients

Objective To investigate the expression of CCKA receptor and its relation with gallbladder hypomotility in patients with gallstone. Methods 20 patients with gallstone and 10 normal subjects were studied and gallbladder emptying function was measured by B ultrasonography. The other 8 patients without gallstone who died of accident were selected as controls for measuring mRNA expression of CCKA receptor of gallbladder; RTPCR was used to explore gene expression of CCKA receptor of gallbladder. Results The gallbladder motility was significantly impaired in gallstone patients. The patients had lower mRNA expression of CCKA receptor than control subjects(06330167 vs. 09440230, P<001), and so did petients with impaired galltb ladde motility then those with normal motility(05440124 vs. 07680131,P<005). Furthermore, the change of expression of CCKA receptors was markedly correlated with gallladder emptying(r=0925,P<001).T5HZConclusions mRNA expression of CCKA receptor in gallstone patients decreases, which was related to galbadder hypomotility. The downregulation of gene expression of CCKA receptor plays an importent role in gallbladder hypomotility in patients with gallstone.