术前介入化疗对直肠癌细胞凋亡和增殖的影响

目的 观察术前介入化疗对直肠癌的疗效及对细胞凋亡和细胞增殖的时相变化的影响。方法 对１２例直肠癌患者术前应用Ｓｅｌｄｉｎｇｅｒ法经皮股动脉穿刺插管,选择肿瘤供血脉一次性化疗药物,５－氟脲嘧啶６００ｍｇ／ｍ＾２,丝裂霉素Ｃ１５ｍｇ／ｍ＾２,阿霉素３５ｍｇ／ｍ＾３。分别于化疗前,化疗后２４、４８、７２小时和７￣１０天取肿瘤组织,应用末端脱氧核苷酸转移酶介导的原位标记法（ＴＵＮＥＬ）检测凋亡细胞,应用免

Timing changes of apoptosis and proliferating cells nuclear antigen after intra-arterial infusion chemotherapy for rectal cancer

OBJECTIVE: To investigate the timing changes of apoptosis (APO) and PCNA after intra-arterial infusion chemotherapy for rectal cancer. METHODS: Twelve patients were subjected to percutaneous arterial femoralis catheterization by Seldinger's technique and infusion of anti-cancer drugs: 5-fluorouracil (5-Fu) 600 mg/m(2), mitomycin (MMC) C5 mg/m(2) and epirubicin (EDR) 35 mg/m(2). The biopsy of rectal tumor tissues was done before chemotherapy, and 24, 48, 72 hours and 7-10 days after chemotherapy. Apoptotic cells were examined by terminal-deoxynucleotidyl-transferase (TdT)-mediated dUTP-fluorescein and labeling. The expression of proliferating cells nuclear antigen (PCNA) was detected by immunohistologic staining. RESULTS: The apoptosis index (AI) of rectal cancer cells before chemotherapy, and 24, 48, 72 hours, and 7-10 days after chemotherapy was 6.84 per thousand, 19.53 per thousand, 13.15 per thousand, 11.85 per thousand and 11.86 per thousand respectively. The PCNA index (PI) was 45.56%, 40.68%, 39. 87%, 51.28% and 63.75% before and 24, 48, 72 hours, and 7-10 days after chemotherapy. CONCLUSIONS: Intra-arterial infusion chemotherapy not only induced apoptosis effectively, but also inhibited temporarily tumor cells proliferation in patients. The curative surgical treatment should be performed as soon as possible after chemotherapy.