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| 高效液相色谱法测定选奇头痛滴丸中黄芩苷的含量 | |
| 引用本文： | 于燕莉,梁爱君,张传霞,毕云生,潘菡青,马晓雯.高效液相色谱法测定选奇头痛滴丸中黄芩苷的含量[J].解放军药学学报,2010,26(2):147-148. |
| 作者姓名： | 于燕莉 梁爱君 张传霞 毕云生 潘菡青 马晓雯 |
| 作者单位： | 1. 济南军区总医院药剂科,山东济南,250031 2. 总后勤部卫生部药品仪器检验所,北京,100071 3. 济南市第五人民医院,门诊部手术室,山东济南,250022 4. 泰山医学院,山东泰安,271016 |
| 摘 要： | 目的建立高效液相色谱法测定选奇头痛滴丸中黄芩苷的含量。方法色谱柱：Chromasil-C18柱（150mm×4.6mm,5μm）;流动相：甲醇-水-磷酸（47∶55∶0.2）;流速：1.0ml/min;检测波长：280nm;柱温：室温。结果黄芩苷在24.8～248μg/ml范围内,进样量与峰面积线性关系良好,r=0.9997（n=6）,平均加样回收率为100.28%,RSD为0.89%。结论本方法操作简便、准确、专属性强、灵敏度高、重复性好,可用于选奇头痛滴丸中黄芩苷的含量测定和质量控制。 |
| 关 键 词： | 选奇头痛滴丸 高效液相色谱 黄芩苷 |
Content Determination of Baicalin in Xuanqitoutong Drop Pills By High Performance Liquid Chromatography |
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| YU Yan-li,LIANG Ai-jun,ZHANG Chuan-xia,BI Yun-sheng,PAN Han-qing,Ma Xiao-wen.Content Determination of Baicalin in Xuanqitoutong Drop Pills By High Performance Liquid Chromatography[J].Pharmaceutical Journal of Chinese People's Liberation Army,2010,26(2):147-148. | |
| Authors: | YU Yan-li LIANG Ai-jun ZHANG Chuan-xia BI Yun-sheng PAN Han-qing Ma Xiao-wen |
| Institution: | (①Department of Pharmcy , General Hospital of Jinan Military Command, Jinan 250031,China;②Institute for Drug and Instrument Control of PLA,Beijing 100071,China;③The Fifth People＇s Hospital, Jinan 250022,China;④Tanshan Medical College,Taian 271016,China ) |
| Abstract: | Objective To establish a method for determination of baicalin in Xuanqitoutong drop pills by high performance liquid chromatography. Methods C18 ODS column（150 mm×4.6 mm,5 μm）was used;the mobile phase consisted of methanol-water-H3PO4（47∶55∶0.2） and the flow rate was 1.0 ml/min,with the wavelength of detection at 280 nm.Results The average recovery of baicalin was 100.28%.The relative standard deviation was 0.89%.The linear range was 24.8-248 μg/ml,（r=0.9997）.Conclusion The method is simple, rapid and sensitive with good repeatability,which can be used for quality control of baicalin in Xuanqitoutong drop pills. |
| Keywords: | high performance liquid chromatography Xuanqitoutong drop pills content determination |
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