| 人早幼粒白血病细胞系HL－60体外诱导分化研究 |
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| 引用本文： | 杨如俊,张明华,严烽,倪奕俊. 人早幼粒白血病细胞系HL－60体外诱导分化研究[J]. 第二军医大学学报, 1994, 0(2) |
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| 作者姓名： | 杨如俊 张明华 严烽 倪奕俊 |
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| 作者单位： | 第二军医大学海医系放射医学研究室 |
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| 摘 要： | 用体外短期液体培养技术，观察ＲＡ和不同来源的造血调控因子对ＨＬ－６０细胞增殖与分化的影响。以生长曲线和倍增时间作为观察各种试剂对ＨＬ－６０细胞增殖作用的指标；以ＮＢＴ还原反应和细胞形态变化作为分化成熟的指标。结果是ＲＡ、ＰＨＡ－ＬＣＭ单用时对ＨＬ－６０细胞均有抑制增殖和诱导分化作用，ＰＷＭ－ＳＣＭ、ＬＰ３－ＣＭ仅部分抑制增殖，而ＦＧＦ、Ｈｕ－ＣＳＦ和ＥＰＯ等无效。各种造血生长因子分别与ＲＡ合用时，能加强ＲＡ对ＨＬ－６０细胞的增殖抑制和诱导分化作用的有ＦＣＦ、ＰＷＭ－ＳＣＭ、ＰＨＡ－ＬＣＭ和Ｈｕ－ＣＳＦ等；ＬＰ３－ＣＭ仅增强抑制增殖：ＥＰＯ未显示作用。
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| 关 键 词： | 造血调控因子；维甲酸；HL－60细胞系；诱导分化 |
The Effects of Retinoic Acid and Hematopoietic Modulation Factors on Induction of Differentiation of HL-60 Cell Line |
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| Yang Rujin, Zhang Minghua, Yan Feng, Ni Yijun. The Effects of Retinoic Acid and Hematopoietic Modulation Factors on Induction of Differentiation of HL-60 Cell Line[J]. Former Academic Journal of Second Military Medical University, 1994, 0(2) |
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| Authors: | Yang Rujin Zhang Minghua Yan Feng Ni Yijun |
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| Abstract: | Using a short-term liquid culture assay, the effects of induction of differentiation of HL-60 cells by retinoic acid (RA) and several hematopoietic modulation factors were evaluated.The results demonstrated that (1) RA and PHA-LCM were capable of inhibiting proliferation, and inducing differentiation of HL-60 cell line, respectively. (2) PWM-SCM and LP3-CM had partial an inhibitory effect on HL-60 cell growth while FGF, Hu-CSF and EPO had no such an effect.(3) although any one of hematopoietic modulation factors by itself had no siginficant effect on induction of differentiation of HL-60 cell line, it could euhance granulocytic maturation when used in combination with RA, except for LP3-CM and EPO. |
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| Keywords: | hematopoietic modulation factor retinoic acid HL-60 cell line induction of differentiation |
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