C5a反义肽对肺血管内皮细胞与中性粒细胞粘附的影响

目的：探讨补体C5a反义肽在C5a过敏毒素介导的肺血管内皮细胞与中性粒细胞粘附中的阻断作用。方法：采用流式细胞术，检测C5a与其反义肽相互作用后再刺激人肺血管内皮细胞(PVEC)，对细胞表面粘附分子P选择素(P-Selectin)表达的影响；同时通过测定粘附于血管内皮细胞表面的PMN中髓过氧化物酶(MPO)活性，间接反映血管内皮细胞表面粘附的PMN数量的变化。结果：发现反义肽R4可以剂量依赖关系抑制C5a介导的PVEC上粘附分子P-Selectin的表达，当R4浓度为5000ng/ml时，可使P-Selectin的表达量下降20％左右，进一步增加R4的浓度，P-Se1ectin表达量无显著性改变；另外，反义肽R4对PVEC与PMN的粘附也有明显抑制作用，表现为PVEC上粘附的PMN中MPO活性的降低，当R4的浓度增加到5000ng/ml，MPO的活性可降低近40％左右。结论：C5a反义肽对C5a过敏毒素的生物学活性具有一定的拮抗作用。

Blocking effects of anti-sense peptide of C5a on the adhesion between pulmonary vascular endothelial cell and neutrophil

Objective:To study the blocking effects of anti-sense peptide of C5a on the adhesion between pulmonary vascular endothelial cell(PVEC) and neutrophil resulting from C5a anaphylatoxin.Methods:It was determined by Flowcytometry that the change of adhesion molecule expression on PVEC and the activity of MPO in PMN was determined after adhesion.Results:In response to C5a after interactions with several concentrations of anti-sense peptide R4, the expression of P-Selectin on PVEC decreased significantly and reached the minimum at the concentration of 5 000 ng/ml, and the activity of MPO in PMN reduced by 40% at the concentration of 5 000 ng/ml of anti-sense peptide R4.Conclusion:The results suggested that anti-sense peptide of C5a has significant blocking effects on C5a anaphylatoxin.