手法对兔膝骨性关节炎软骨细胞DNA氧化性损伤的防治作用

为研究手法对膝关节骨性关节炎治疗的作用机制,采用新西兰兔进行动物造模而形成膝关节骨性关节炎模型,随机将实验动物分为手法组、施沛特组和对照组并进行实验。8周后取动物右膝关节软骨进行DNA提取,并采用LCMS2010型液-质联用仪检测标本中8-羟基脱氧鸟苷含量。结果显示,施沛特组含量最少,手法组次之,对照组最多。提示手法治疗可能对减轻氧自由基对软骨细胞DNA的损伤有一定的作用。

PREVENTIVE AND THERADEUTIC EFFECT OF MANIPULATIONS ON CHONDROCYTE DNA OXYDIZING INJURY IN RABBIT KNEE OSTEOARTHRITIS

In order to study the action mechanism of the manipulations in the treatment of knee osteoarthritis(KOA),new Zealand rabbits were made into KOA models and randomly divided into three groups: Group A,Group B and Group C.Group A and Group B were treated by the manipulations and by intra-articular injection of Sodium Hyaluronate,respectively and Group C gave no treatment;eight weeks later,the DNA was extracted from the right-knee cartilage and the 8-OHDG contents in the samples were measured by LCMS2010 apparatus.The results showed that the 8-OHDG content of Group B was the lowest,the one of Group A was the second and the one of Group C was the most,suggesting that the manipulations could have a certain function to alleviate the chondrocyte DNA injury by oxygen free radical.