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| 脱细胞猪角膜基质体外支持角膜上皮和基质细胞的生长 | |
| 引用本文： | 林旭初,惠延年,孟浩,张永杰,金岩.脱细胞猪角膜基质体外支持角膜上皮和基质细胞的生长[J].国际眼科杂志,2008,8(7):1293-1295. |
| 作者姓名： | 林旭初 惠延年 孟浩 张永杰 金岩 |
| 作者单位： | 1. 530021,中国广西壮族自治区南宁市,解放军303医院眼科;710032,中国陕西省西安市,第四军医大学组织工程研发中心;710032,中国陕西省西安市,第四军医大学西京医院眼科 2. 第四军医大学西京医院眼科,中国陕西省西安市,710032 3. 第四军医大学组织工程研发中心,中国陕西省西安市,710032 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 目的:探讨脱细胞猪角膜基质体外能否支持兔角膜细胞的生长。方法:体外培养兔角膜上皮细胞和基质细胞,并接种到制备的脱细胞猪角膜基质上,倒置相差显微镜和组织学观察细胞生长情况。结果:上皮细胞能在脱细胞猪角膜基质上贴附生长,10d时可形成2~3层的复层结构。基质细胞在脱细胞猪角膜基质上贴附生长后可向材料深层迁徙。结论:制备的脱细胞猪角膜基质体外可支持兔角膜上皮细胞和基质细胞的生长。 |
| 关 键 词： | 脱细胞猪角膜基质 角膜上皮 角膜基质细胞 组织工程 |
Support of acellular porcine corneal stroma for growth of corneal epithelium and stromal cell in vitro |
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| Xu-Chu Lin,Yan-Nian Hui,Hao Meng,Yong-Jie Zhang,Yan Jin.Support of acellular porcine corneal stroma for growth of corneal epithelium and stromal cell in vitro[J].International Journal of Ophthalmology,2008,8(7):1293-1295. | |
| Authors: | Xu-Chu Lin Yan-Nian Hui Hao Meng Yong-Jie Zhang Yan Jin |
| Institution: | 1Department of Ophthalmology, No. 303 Hospital of Chinese PLA, Nanning 530021, Guangxi Zhuang Autonomous Region, China2Research and Development Center for Tissue Engineering, School of Stomatology, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China3Department of Ophthalmology, Xijing Hospital, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China |
| Abstract: | AIM:To determine whether acellular porcine cornea stroma (APCS) could support the growth of the rabbit corneal cells in vitro.METHODS: APCS was prepared. The rabbit's corneal epithelium and stromal cells were cultured and seeded on, APCS in vitro.The observation of phase contrast photograph and histological examination were performed.RESULTS: Histological examination showed the epithe- lium grew on the scaffold of APCS in 2-3 layers at 10th day. The stromal cells adhered to the surface of the scaffold after 24 hours and invaded into the interlaminar of the material at 5th day.CONCLUSION: These results indicate that APCS can support the growth and proliferation of the corneal epithelium and stromal cells in vitro. |
| Keywords: | acellular porcine cornea stroma corneal epithelium corneal stromal cells tissue engineering |
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