水半夏组培快繁体系的建立

目的建立水半夏的组培快繁体系,为快速、大量生产水半夏种苗提供基础。方法利用不同植物激素配比的培养基,以水半夏块茎为外植体进行试验。结果愈伤培养基MS+NAA 0.2 mg/L+KT 1.0 mg/L+蔗糖3%+琼脂6 g/L可成功诱导水半夏愈伤组织;丛生芽培养基MS+NAA 0.2 mg/L+6-BA 2.0 mg/L+蔗糖3%+琼脂6 g/L诱导不定芽效果较好,增殖倍数高;生根培养基1/2 MS+NAA 0.2 mg/L+IBA 0.4 mg/L+KT 0.2 mg/L+蔗糖3%+琼脂6 g/L+AC 0.3 g/L,有利于水半夏不定根的快速形成,12～14周即可获得再生水半夏植株。培养基MS+NAA 0.2 mg/L+6-BA 1.5 mg/L+蔗糖3%+琼脂6 g/L为诱导水半夏分化一次性成苗的最佳激素配比,5～6周可形成试管苗,10周后可用于炼苗。结论建立的水半夏组培快繁体系为水半夏优良品种的快速繁殖奠定基础,且可应用于工厂化生产水半夏种苗。

Establishment of tissue culture and rapid propagation system of Typhonium flagelliforme

Objective To establish the rapid propagation system and tissue culture techniques of Typhonium flagelliforme for large-scale seedlings.Methods Using media with different hormones proportions,the tuber tissue was found to be a good explant for inducing asexual propagation system.Results The callus culture medium consisted of MS+NAA 0.2 mg/L+KT 1.0 mg/L+sucrose 3%+agar 6 g/L could generate callus successfully.Medium consisted of MS+NAA 0.2 mg/L+6-BA 2 mg/L+sucrose 3% + agar 6 g/L was a superlative proportion of hormones concentration to generate adventitious buds efficiently,especially for higher proliferated multiples.The rooting medium consisted of 1/2 MS+NAA 0.2 mg/L+IBA 0.4 mg/L+KT 0.2 mg/L+sucrose 3%+agar 6 g/L+AC 0.3 g/L was conducive to generate roots rapidly.The explants could be acclimatized after a period of 12 to 14 weeks.Experiments of one-step-seedling formation indicated that the one-step-seedling formation medium,containing MS+NAA 0.2 mg/L+6-BA 1.5 mg/L+sucrose 3%+agar 6 g/L was the best proportion of hormone concentration,for 5 to 6 weeks,new plantlets could be developed,which will be acclimatized in 10 weeks.Conclusion The tissue culture techniques and rapid propagation system of T.flagelliforme could be used for large-scale seedlings and lay a foundation for its improved breeds.