HLA-DQB1基因多态性与2型糖尿病的关系

目的:探讨中国北方汉族人HLA-DQB1等位基因多态性与2型糖尿病的关系.方法:采用PCR技术和限制性内切酶片段长度多态性分析方法对49例2型糖尿病患者及55例正常对照者进行了HLA-DQB1基因分析.结果:病例组和对照组HLA-DQB1基因突变型等位基因(G)频率在病例组和对照组间差异无显著性(χ2=0.36, P>0.05),GG、GC 、CC三种基因型出现频率差异无显著性.基因型频率的相对风险分析结果表明 GC基因型与CC基因型患2型糖尿病风险度为1.02, GG基因型与CC基因型患2型糖尿病风险度为0.43.结论:HLA-DQB1基因突变型等位基因与2型糖尿病发病无必然的联系,突变基因型并没有增加2型糖尿病的发病风险.

Relationship between polymorphism of HLA-DQB1 alleles and type 2 diabetes mellitus

Objective:To explore the potential association between HLA DQB1 allele polymorphism and type 2 diabetes (non insulin dependent diabetes mellitus,NIDDM) in Chinese north Han people. Methods:PCR restrictive fragment length polymorphism (RFLP) typing was used to identify HLA DQB1 alleles in the group of patients with NIDDM ( n =49) and health controls ( n =55). Results: Compared with normal controls, the allelic frequency of HLA DQB1 mutation was no significantly different in NIDDM group with 0.827 relative risk (RR) ( χ 2=0.36, P >0.05), and the frequencies of the three HLA DQB1 genotypes were not significantly different between the case and the control. The relative risk suffering from NIDDM of GC genotype was 1.02 times of the CC genotype, and the GG genotype was 0.43 times of the CC genotype. Conclusion: These results showed that susceptibility to NIDDM is not correlated with HLA DQB1 gene. The mutant gene did not increase the risk of suffering from NIDDM.