| 下调CXCR4表达对胶质瘤干细胞侵袭能力和VEGF分泌量的影响 |
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| 引用本文： | 赵林涛,卞修武,姜建勇,王斌,平轶芳,余时沧.下调CXCR4表达对胶质瘤干细胞侵袭能力和VEGF分泌量的影响[J].第三军医大学学报,2009,31(11). |
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| 作者姓名： | 赵林涛 卞修武 姜建勇 王斌 平轶芳 余时沧 |
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| 作者单位： | 第三军医大学西南医院病理学研究所,重庆,400038 |
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| 摘 要： | 目的 观察下调胶质瘤干细胞CXCR4表达后对其侵袭能力和血管内皮生长因子(vascular endothelial growth factor,VEGF)分泌量的影响.方法 采用间接免疫荧光标记观测U87细胞及其颅内移植瘤标本中胶质瘤干细胞标记物CD133和CXCR4共表达情况;分别从稳定转染CXCR4小干扰RNA质粒的U87细胞和稳定转染阴性对照质粒的U87细胞中培养出胶质瘤干细胞球,并对其进行鉴定,观察两者CXCR4的表达情况;通过Transwell小室比较两者的侵袭能力;采用酶联免疫吸附实验(ELISA)检测两者细胞培养上清内VEGF的含量.结果 U87细胞及其移植瘤中均可检测到CXCR4与CD133共表达,下调胶质瘤干细胞CXCR4表达后,胶质瘤干细胞的体外侵袭能力明显下降干扰组:(17.0±5.8),对照组:(71.5±8.7),P＜0.05],VEGF分泌明显减少干扰组:24 h(690±24)pg/ml,48 h(1 504±53)pg/ml;对照组:24 h(850±25)pg/ml,48 h(2 001±150)pg/ml,P＜0.05].结论 胶质瘤干细胞表达CXCR4,下调其表达可抑制胶质瘤干细胞的侵袭和促血管生成能力,提示CXCR4可能成为针对胶质瘤干细胞进行治疗的靶点.
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| 关 键 词： | 胶质瘤干细胞 侵袭 血管内皮生长因子 血管生成 |
Down-regulation of CXCR4 expression inhibits invasion and VEGF production of glioma stem cells |
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| ZHAO Lin-tao,BIAN Xiu-wu,JIANG Jian-yong,WANG Bin,PING Yi-fang,YU Shi-cang.Down-regulation of CXCR4 expression inhibits invasion and VEGF production of glioma stem cells[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(11). |
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| Authors: | ZHAO Lin-tao BIAN Xiu-wu JIANG Jian-yong WANG Bin PING Yi-fang YU Shi-cang |
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| Institution: | ZHAO Lin-tao,BIAN Xiu-wu,JIANG Jian-yong,WANG Bin,PING Yi-fang,YU Shi-cang(Institute of Pathology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To observe the effects of CXCR4 downregulation on the invasion capacity and VEGF secretion of glioma stem cells(GSCs).Methods Coexpression of CXCR4 and CD133 in U87 cells and their xenografts grown in nude mice brain was detected by indirect immunofluorescence,under a laser scanning microscope.Tumorspheres were isolated from control U87 cells and CXCR4-siRNA U87 cells,respectively.We investigated the expression of CD133,nestin and CXCR4 in tumorspheres by laser scanning microscopy.Effects of CXCR4... |
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| Keywords: | CXCR4 |
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