| 急性肝衰竭大鼠胃肠运动改变与肠神经元关系研究 |
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| 引用本文： | 许伟红,刘梅,吕志武,陈煜,郑素军,刘旭华,赵军,丁美,段钟平. 急性肝衰竭大鼠胃肠运动改变与肠神经元关系研究[J]. 实用肝脏病杂志, 2011, 14(1): 3-6,10. DOI: 10.3969/j.issn.1672-5069.2011.01.002 |
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| 作者姓名： | 许伟红 刘梅 吕志武 陈煜 郑素军 刘旭华 赵军 丁美 段钟平 |
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| 作者单位： | 1. 哈尔滨医科大学附属第二医院消化内科,哈尔滨市,150086
2. 北京首都医科大学附属北京佑安医院人工肝中心 |
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| 基金项目： | 北京市教育委员会科技发展计划项目(KM201010025024); “艾滋病和病毒性肝炎等重大传染病防治”科技重大专项课题(2008ZX10002-005-3); 国家自然科学基金课题(30800979,30800517); 北京市科技新星项目(2007B055) |
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| 摘 要： | 目的观察急性肝衰竭大鼠胃肠消化间期移行性复合运动的变化与肠神经元的关系。方法采用D-氨基半乳糖诱导的急性肝衰竭大鼠模型,使用多道生理记录仪记录正常大鼠和急性肝衰竭大鼠的胃肠消化间期移行性复合运动,采用TUNEL法、5-HT和NSE免疫组化法检测空肠神经元的凋亡情况。结果急性肝衰竭大鼠空肠MMC周期较对照组显著延长（1897.71±815.77对1384.17±449.34S,P〈0.05）,尤以Ⅱ期延长为主（1870.90±1010.35S对643.04±450.67S,P〈0.05）,成簇状移行性收缩,Ⅲ期显著缩短（31.41±11.17S对53.11±14.74S,P〈0.05）;急性肝衰竭大鼠肠神经元凋亡明显增多,TUNEL染色阳性细胞核固缩或碎裂,呈棕黄色,细胞大小不一;5-HT和NSE免疫组化检测发现急性肝衰竭大鼠阳性物质表达较正常大鼠减少。结论急性肝衰竭大鼠胃肠MMCⅡ期延长,Ⅲ期明显缩短,导致胃肠动力异常,可能与肠神经元的凋亡有关。
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| 关 键 词： | 急性肝衰竭 移行性复合运动 肠神经元 |
The relationship between enteric neuronal and gastrointestinal motility in rats with acute liver failure |
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| XU Weihong,LIU Mei,LV Zhiwu,et al.. The relationship between enteric neuronal and gastrointestinal motility in rats with acute liver failure[J]. Journal of Clinical Hepatology, 2011, 14(1): 3-6,10. DOI: 10.3969/j.issn.1672-5069.2011.01.002 |
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| Authors: | XU Weihong LIU Mei LV Zhiwu et al. |
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| Affiliation: | XU Weihong,LIU Mei,LV Zhiwu,et al.Department of Gastroenterology,Second Affiliated Hospital,Haerbin Medical University,Haerbin 100086,China |
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| Abstract: | Objective This study was undertaken to explore the relationship between enteric neuronal and migrating motor complexes(MMC)in rats with acute liver failure.Methods Wistar rats were given the D-galactosamine hydrochloride(D-Gal)to induce acute liver failure.Then,the MMC was recorded by a multilead physiologival recording system.TUNEL,5-HT and NSE immunohistochemistry were used to detect neuronal apoptosis.Results The duration of jejunal MMC was significantly prolonged in rats with acute liver failure compare... |
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| Keywords: | Acute liver failure Migrating motor complex Enteric neuronal |
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