藻酸双酯钠对类缺血再灌注神经元NF-κB表达的影响

 目的研究藻酸双酯钠对神经元类缺血再灌注损伤的NF-κB表达的影响。方法 利用免疫细胞化学法应用β-Tubulin及Gap-43对培养的皮质神经元进行鉴定，利用流式细胞仪对经藻酸双酯钠处理后的培养的类缺血再灌注损伤后的皮质神经元NF-κB表达情况进行研究。结果 ①在培养的神经元上β-Tubulin及Gap-43表达大概为80%左右；②再灌注30 min时，藻酸双酯钠浓度从0.01～0.1 mg·mL^-1时，检测的NF-κB阳性细胞比率明显增加，当藻酸双酯钠浓度达1 mg·mL^-1时，阳性细胞数明显减少。结论不同浓度的藻酸双酯钠对培养的小鼠皮质神经元在类缺血再灌注时NF-κB的表达有较大影响，小剂量时有促进作用，大剂量时有抑制作用。

Effects of polysaccharide sulfate on the expression of NF-κB in cortical neurons in ischemic like condition

OBJECTIVE To explore the effects of polysaccharide sulfate(PSS) on the expression of NF-κB in cortical neurons in ischemic like condition. METHODS The cortical neurons were identified by β-Tubulin and Gap-43 by means of immunocytochemistry.The cortical neurons were treated with different densities of PSS.The expression of NF-κB was indetected by flow cytometer. RESULTS The ratio of positive neurons expressing β-Tubulin and Gap-43 was 80%.The positive neurons expressing NF-κB was increased following the increase of PSS concentrations from 0.01 to 0.1 mg·mL^-1.The positive neurons expressing NF-κB was decreased when the concentrations of PSS increased from 0.01 to 0.1 mg·mL^-1.CONCLUSION The expression of NF-κB in cortical neurons in ischemic like condition was affected by the density of PSS.