Oxotremorine antagonism by prolyl-leucyl-glycine-amide administered by different routes and with several anticholinergics

Prolyl-leucyl-glycine amide (PLG) was given by oral, intravenous, or subcutaneous routes of administration and shown to antagonize the central and peripheral effects of oxotremorine. The previously demonstrated action of PLG in this system when injected intraperitoneally in a single dose was found to be unchanged when administered by the same route for five consecutive days. Although three anticholinergic compounds were also effective in reducing the effects of oxotremorine, no interactions between PLG and trihexyphenidyl hydrochloride, benzotropin mesylate, or scopolamine were observed. The results confirm and extend the demonstration of the extra-endocrine effects of PLG.