栗酮对动脉粥样硬化大鼠的治疗作用

目的：观察栗酮对动脉粥样硬化大鼠的治疗作用。方法：采用高脂饲料喂养的方法诱发大鼠动脉粥样硬化模型。造模8周后,选取造模成功大鼠50只随机分为栗酮300、150、75mg/kg剂量组、阳性组（非诺贝特）、模型对照组,每组10只,连续灌胃给药60d,模型对照组给予等体积的蒸馏水,给药同时仍采取高脂饲料喂养。另取10只大鼠为正常对照组,灌胃给予蒸馏水,正常饲料喂养。60d后分别采血检测血清总胆固醇（CHOL）、TG、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、氧化型低密度脂蛋白（Ox-LDL）及取胸主动脉内皮细胞观察细胞凋亡情况,同时取主动脉作病理形态学观察。结果：大鼠连续给栗酮（300mg/kg、150mg/kg）60d血清CHOL、TG、LDL-C、Ox-LDL、细胞凋亡比率较模型对照组明显减低（P〈0.05）,300mg/kg、150mg/kg剂量组大鼠主动脉壁泡沫细胞数目及弹性纤维损伤等病变明显较高脂模型组减轻。结论：栗酮具有明显的降脂及抗动脉壁损伤的作用。

Study on therapeutic effect of LITONG on atherosclerosis rats

AIM：To observe the therapeutic effect of LITONG on atherosclerosis rats.METHODS：The atherosclerosis rat models were induced by feeding high fat diet.After 8 weeks,60 rats were selected and were randomly divided into six groups：300 mg/kg,150 mg/kg,75 mg/kg of LITONG,positive drug control（Fenofibrate）,model control and normal rat control.The rats in model groups were introgastricly administrating distilled water and were fed high fat diet,and the rats in normal group were introgastricly administrating distilled water.After 60 days,the levels of serum total cholesterol,triglycerides,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol were detected by blood samples.In addition,the aorta pathology alteration and cell apoptosis of arterial wall endothelial were also examined.RESULTS：Significant differences were seen in the levels of total cholesterol,triglyceride,low-density lipoprotein cholesterol and cell apoptosis ratio in LITONG groups （ 300 mg/kg,150 mg/kg ） compared with those in model group（P〈0.05）.In addition,the number of foam cells in rat aortic wall and the extent of elastic fiber damage reduced significantly in high-and low-dose groups of LITONG compare with those in model group.CONCLUSION：These studies suggest that LITONG has satisfactory pharmacologic effects on atherosclerosis and can improve elastic fiber damage of rats.