荧光定量PCR技术对1235例NGU病原体基因检测结果分析

目的用实时荧光定量PCR（FQ—PCR）技术准确定量检测非淋菌性尿道炎（NGu）中解脲支原体（UU）、沙眼衣原体（CT）基因，进一步了解UU、CT感染情况，为临床治疗提供依据。方法采用FQ—PCR技术检测标本1235份。结果UU阳性率是27．5％，其中男性UU阳性率是12．4％，女性UU阳性率是42．0％。CT阳性率是12．1％，其中男性CT率是11．2％，女性CT率是13．2％。UU感染率在男性、女性患者有极显著性差异（P〈0．01），而CT感染率在性别之间无显著性差异（P〉0．05）。结论实时荧光定量PCR检测UU、CT基因具有操作简单、反应时间短、重复性好、结果客观准确、敏感性和特异性好等优点，其结果对临床诊断、治疗有一定指导意义。

Detection and Analysis of the gene of causative agents of 1235 cases of non-gonococcal urethritis by fluorescence quantitative PCR

Objective To detect quantitatively the gene of Ureaplasm urealyticum （UU） and Chlamydia trachomatis （CT） in non--gonococcal urethfitis by technology of fluorescence quantitative polymerase chain reaction （FQ-PCR）,to understand further infections condition of UU and CT ,and to provide clinical treatment evidence to the disease. Methods Detection 1235 speciments by technology of FQ-PCR. Results the positive of UU was 27.5% of all these;male posiaive was 12.4%, and female was 42.0%0 the positive of CT was 12.1% of all these male posiaive was 11.2% ,and female was 13.2%. Infectious rates of UU had obvious statistics difference between males and females,but CT hadn＇t. Conclusions The fluorescence quantitative PCR assays showed advantages which were mainpulated simply,time--saving,more extra,more sensitive and more spectific in detecting CT and UU. FQ-PCR had directive meaning for dagnosis and treatmeat.