首页 | 本学科首页   官方微博 | 高级检索  
检索        

低蛋白饮食对环孢素A肾病大鼠肾间质纤维化的作用
引用本文:徐志宏,谌贻璞,唐功耀,董鸿瑞.低蛋白饮食对环孢素A肾病大鼠肾间质纤维化的作用[J].中华肾脏病杂志,2007,25(1):465-470.
作者姓名:徐志宏  谌贻璞  唐功耀  董鸿瑞
作者单位:卫生部中日友好医院肾病中心,北京,100029;
摘    要:Objective To investigate whether low-protein diet has protective effect on the progression of renal interstitial fibrosis in rats with cyclosporine A (CsA)-induced nephropathy. Methods Eighteen male Sprague-Dawley rats were randomly divided into three groups (6 rats in each group). The rats in control group (C group) received common diet; in model group (M group) low-salt diet; in intervention group (Ⅰ group) low-salt and low-protein diet. After diet adaptation period of one week, the rats in C group received subcutaneous injection of olive oil 1 mg/kg daily for 5 weeks, while M group and Ⅰ group subcutaneous injection of CsA (diluted into 25 g/L with olive oil) 1 ml/kg for 5 weeks. All the rats were sacrificed at the end of the 5th week. The food-intake and body weight were measured daily. The creatinine clearance (Ccr) was examined before rats were sacrificed. The semi-quantitative pathological analysis on kidney sections was performed. The mRNA and protein expression of transforming growth factor-β1 (TGF-βI) and type Ⅰ collagen (Col Ⅰ) in kidney tissue was determined with real time PCR and immunohistochemical staining, respectively. Results The food-intake and body weight of rats in M and I groups were significantly lower than those in C group (P<0.05). Compared with C group, the Ccr levels in M and Ⅰ groups were significantly reduced (0.65±0.15) ml/min, (0.40+0.13) ml/min vs (1.55±0.29) ml/min, P<0.05], the relative fibrosis areas of kidney interstitium in M and I groups were significantly increased (3.60%±0.46%, 3.26%±0.75% vs 0.44%±0.24%, P<0.05), the mRNA and protein expression of TGF-β1 in M and I group was significantly up-regulated (by 2.6 and 3.1 times in mRNA and by 1.5 and 1.6 times in protein, respectively, P<0.05), and the mRNA and protein expression of Col Ⅰ in M and I groups was also significantly up-regulated (by 3.0 and 3.5 times in mRNA and by 2.3 and 2.1 times in protein, respectively, P<0.05). There were no significant differences between M and I groups in every parameters above-mentioned except the rat body weight and Ccr. Both the body weight and Ccr in Ⅰ group were significantly lower than those in M group (P<0.05). Compared with C group, the urine osmotic pressure in M group and in I group were deceased (for M group, P>0.05; for I group, P<0.05). Compared with C group, the serum cholesterol levels in M and I groups were significantly increased (P<0.05), and the serum phosphorus level in I group was significantly decreased (P<0.05). The levels of serum albumin and serum calcium of all three groups had no statistical differences (P>0.05). Conclusion Low-protein diet has no renoprutective effects on the rat model of cyclosporin A nephropathy, on the contrary, may induce body weight loss.

关 键 词:膳食  限制蛋白质    环孢菌素    纤维化    转化生长因子β1    胶原Ⅰ型    Collagen  type      

Effect of low protein diet on the progression of renal interstitial fibrosis in rats with cyclosporin A nephropathy
XU Zhi-hong,CHEN Yi-pu,TANG Gong-yao,DONG Hong-rui.Effect of low protein diet on the progression of renal interstitial fibrosis in rats with cyclosporin A nephropathy[J].Chinese Journal of Nephrology,2007,25(1):465-470.
Authors:XU Zhi-hong  CHEN Yi-pu  TANG Gong-yao  DONG Hong-rui
Abstract:
Keywords:Diet  protein-restrictedCyclosporineFibrosisTransforming growth factor betal
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号