| 脂质体包裹99mTc-HYNIC-Survivin ASODN的鸟嘌呤含量与启动子位置对显像质量的影响 |
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| 引用本文： | 贾红丽,冯珏,张秀梅,杨阳,李晓东.脂质体包裹99mTc-HYNIC-Survivin ASODN的鸟嘌呤含量与启动子位置对显像质量的影响[J].中国医学影像技术,2011,27(3):449-452. |
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| 作者姓名： | 贾红丽 冯珏 张秀梅 杨阳 李晓东 |
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| 作者单位： | 河北医科大学第二医院核医学科,河北,石家庄,050000 |
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| 摘 要： | 目的 制备鸟嘌呤(G)含量及离启动子远近不同的4种脂质体包裹99mTc-HYNIC-Survivin ASODN对荷人肝癌裸鼠行SPECT显像,探讨其显像质量是否受G含量及启动子位置影响,以找到最佳反义显像的碱基序列,提高基因显像质量。方法 制备两种(A1、A2)G含量不同而离启动子远近相近的16个碱基单链ASODN和两种(A'1、A'2)G含量相同而离启动子远近不同的10个碱基单链ASODN,耦联HYNIC,99mTc标记后,经Cellufine GH-25纯化后脂质体包裹。用此显像剂对荷瘤裸鼠行肿瘤反义显像并通过ROI技术进行分析。结果 肿瘤/对侧肌肉比值(T/NT)A1、A2分别为4.13±0.09 和2.47±0.04(2 h),4.90±0.11和3.75±0.10(4 h);A'1、A'2分别为3.90±0.07和2.09±0.07(2 h),4.41±0.08和2.78±0.10(4 h)。上述各组T/NT值在各时间点差异有统计学意义(P＜0.01)。结论 G含量相同,离启动子位置较近,显像质量较好;离启动子位置相近,G含量较低,显像质量较好。
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| 关 键 词： | 寡核苷酸 反义 启动区域(基因) 鸟嘌呤 放射性核素显像 |
| 收稿时间： | 2010/7/28 0:00:00 |
| 修稿时间： | 2010/8/19 0:00:00 |
Imaging quality influenced by liposome-coated 99mTc-HYNIC-Survivin ASODN's guanine content and promoter's position |
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| JIA Hong-li,FENG Jue,ZHANG Xiu-mei,YANG Yang and LI Xiao-dong.Imaging quality influenced by liposome-coated 99mTc-HYNIC-Survivin ASODN's guanine content and promoter's position[J].Chinese Journal of Medical Imaging Technology,2011,27(3):449-452. |
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| Authors: | JIA Hong-li FENG Jue ZHANG Xiu-mei YANG Yang and LI Xiao-dong |
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| Institution: | Department of Nuclear Medicine, the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China;Department of Nuclear Medicine, the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China;Department of Nuclear Medicine, the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China;Department of Nuclear Medicine, the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China;Department of Nuclear Medicine, the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China |
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| Abstract: | Objective To find whether imaging quality of 99mTc-HYNIC-Survivin ASODN in nude mice bearing human hepatocellular carcinoma was influenced by their guanine content and promoter's position using four different kinds of synthesized and liposome-coated 99mTc-HYNIC-Survivin ASODN,and to find optimal antisense imaging sequence for improving the quality of genic imaging.Methods Two kinds(A1,A2) of synthesized Survivin ASODN with two guanine contents(12.5%,50.0%) and close promoter's position,16 bases per single-s... |
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| Keywords: | Oligonucleotides antisense Promoter regions (genetic) Guanine Radionuclide imaging |
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