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Sensitivity of the hormone dependent MXT-mouse mammary carcinoma to estradiol during tumoral growth. An autoradiographic study
Affiliation:1. The Royal (Dick) School of Veterinary Studies and The Roslin Institute, University of Edinburgh, Easter Bush, Edinburgh EH25 9RG, United Kingdom;2. Center for Research and Technology of Agro-Environment and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal;1. Department of Gastroenterology and Hepatology, The First Clinical Hospital of Shanxi Medical University, Taiyuan 030001, China;2. Experimental Center of Science and Research, The First Clinical Hospital of Shanxi Medical University, Taiyuan 030001, China;3. Key Laboratory of Cell Physiology, Department of the Ministry of Education, Shanxi Medical University, Taiyuan 030001, China;4. Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network, Ontario, Canada;5. Division of Cardiac Surgery, Department of Surgery, University of Toronto, Ontario, Canada
Abstract:The MXT transplantable mammary tumor is an ovarian sensitive carcinoma of the B6D2F1 mouse strain. The present work documents the mitogenic effect of a single near-physiological dose (pulse) of estradiol (E2) in castrated mice bearing MXT neoplasms 2–7 weeks after their inoculation. Histological sections of tumors and uteri (as control E2 target organs) were processed and nuclear thymidine labeling indices (TLI) were determined by autoradiography. Tumor estrogen receptors contents were evaluated in parallel. The results indicate that the MXT tumor keeps a constant sensitivity to E2 6 weeks after implantation.
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