| 尿微量白蛋白ELISA测定法及其临床应用 |
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| 引用本文： | 张桂生,刘国明.尿微量白蛋白ELISA测定法及其临床应用[J].现代免疫学,1993,13(5):299-302. |
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| 作者姓名： | 张桂生 刘国明 |
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| 作者单位： | 上海第二医科大学科技中心实验室，上海第二医科大学科技中心实验室，上海第二医科大学科技中心实验室，上海第二医科大学科技中心实验室 上海 200025，上海 200025，上海 200025，上海 200025 |
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| 摘 要： | 定期监测糖尿病患者的尿微量白蛋白,对尚处于可逆性阶段糖尿病性肾病的早期诊断具有重要价值。尿白蛋白也是高血压病引起血管损伤及肾血管早期病变的敏感指标。我们自制高效价抗人Alb血清、抗(人Alb)IgG-HRP酶标记结合物和抗(人Alb)IgG包被微孔滴定板,建立了一种灵敏、简便的尿微量白蛋白酶联免疫测定法,测定范围为5～500ng/ml;灵敏度0.76ng;批内CV％ 6.4％;批间CV％ 16.4％。24小时尿白蛋白排泌量,正常人为8.86(SD 9.23)mg/24h(n=80);糖尿病患者为40.4(SD 40.4)mg/24h(n=16);Ⅰ、Ⅱ、Ⅲ期高血压病患者分别为15.17(SD 14.42)mg/24h、21.46(SD 24.34)mg/24h和24.6(SD 18.9)mg/24h。
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| 关 键 词： | 尿微量白蛋白 酶免疫测定法 糖尿病 高血压病 |
ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA) FOR MICROALBUMINURIA AND ITS CLINICAL USE |
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| Zhang Guisheng Liu Guoming Wang Xinhong Sun min Central Laboratory,Shanghai Second Medical University,Shanghai.ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA) FOR MICROALBUMINURIA AND ITS CLINICAL USE[J].Current Immunology,1993,13(5):299-302. |
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| Authors: | Zhang Guisheng Liu Guoming Wang Xinhong Sun min Central Laboratory Shanghai Second Medical University Shanghai |
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| Institution: | Zhang Guisheng Liu Guoming Wang Xinhong Sun min Central Laboratory,Shanghai Second Medical University,Shanghai 200025 |
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| Abstract: | Periodic monitoring of microalbumin uria could be used to detect diabetic nephro-pathy at early, potentially reversible state. The presence of urinary Alb might also be an early sensitive indicator of renal vascular injury due to high blood pressure. We developed a method for ELISA of microalbuminuria. A high titer anti-human Alb serum,anti(h Alb)IgG-HRP conjugate and anti(h Alb)IgG coated microplate were all developed in our laboratory. The method was sensitive and simple. The assay range was 5-500ng/ml and sensitivity was 0.76ng of albumin. The intraassay and inter-assay CV% were 6.4% and 16.4% respectively. Albumin excretion values were 8.86 (SD 9.23)mg/24h in healthy subjects (n = 80) and 40.4(SD 40.4)mg/24h in diabetics (n= 16). Albumin excretion values were 15.17(SD 14.42)mg/24h,21.46(SD 24.34)mg/ 24h and 24.6(SD 18.9)mg/24h in patients with essential hypertension of I, II, III stages (WHO criteria) respectively. |
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| Keywords: | Microalbuminuria ELISA diabetics essential hypertention |
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