| 荧光原位杂交技术在急性髓系白血病诊断中的应用 |
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| 引用本文： | 许戟,李建勇,陈丽娟,仇海荣,钱思轩,朱雨,李丽.荧光原位杂交技术在急性髓系白血病诊断中的应用[J].实用临床医药杂志,2006,10(9):16-18. |
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| 作者姓名： | 许戟 李建勇 陈丽娟 仇海荣 钱思轩 朱雨 李丽 |
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| 作者单位： | 南京医科大学第一附属医院,江苏省人民医院,血液内科,江苏,南京,210029 |
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| 基金项目： | 江苏省135工程医学重点人才资助项目(RC2002044) |
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| 摘 要： | 目的探讨荧光原位杂交技术(FISH)在急性髓系白血病(AML)的诊断中的意义。方法对初发的经骨髓常规形态学、细胞化学染色和免疫分型初步诊断的10例AML-M2、19例AML-M3,11例AML不能确定为M2或者M3的患者,进行FISH技术检测AML1/ETO和/或PML/RARα融合基因,进而协助诊断和指导治疗。结果10例AML-M2中AML1/ETO融合基因阳性率40%(4/10);19例AML-M3中PML/RARα融合基因阳性率89%(17/19),1例AML1/ETO融合基因阳性,确诊为AML-M2;11例AML中AML1/ETO融合基因阳性率18%(2/11),PML/RARα融合基因阳性率45%(5/11),其余4例未检测到以上两种融合基因。结论FISH是一种敏感的分子遗传学的新技术,具有高效、快速、灵敏等优点。对AML的诊断分型具有重要帮助,进一步指导临床治疗。
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| 关 键 词： | 急性髓系白血病 荧光原位杂交技术 AML1/ETO融合基因 PML/RARα融合基因 |
| 文章编号： | 1672-2353(2006)05-0016-03 |
| 修稿时间： | 2006年3月26日 |
VALUE OF FLUORESCENCE IN SITU HYBRIDIZATION IN DIAGNOSING ACUTE MYELOID LEUKEMIA |
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| XU Ji,LI Jian-yong,CHEN Li-juan,QIU Hai-rong,QIAN Si-xuan,ZHU Yu,LI Li.VALUE OF FLUORESCENCE IN SITU HYBRIDIZATION IN DIAGNOSING ACUTE MYELOID LEUKEMIA[J].Journal of Clinical Medicine in Practice,2006,10(9):16-18. |
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| Authors: | XU Ji LI Jian-yong CHEN Li-juan QIU Hai-rong QIAN Si-xuan ZHU Yu LI Li |
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| Abstract: | Objective To investigate the value of fluorescence in situ hybridization(FISH) technique in the diagnosis of acute myeloid leukemia(AML).Methods FISH was used to detect the AML1/ETO fusion gene and/or PML/RARαfusion gene in 10 AML-M_2 cases,19 AML-M_3 cases and 11 AML cases undetermined as AML-M_2 or AML-M_3 by routine morphology,cytochemical staining and immunophenotyping.Results 4 of 10 AML-M_2 cases were AML1/ETO positive.Among 19 AML-M_3 cases,17 were PML/RARαpositive.1 case was detected AML1/ETO fusion gene.In 11 unconfirmed M_3 or M_2,2 cases showed AML1/ETO and 5 showed PML/RARαfusion gene.Conclusion FISH is a rapid,accurate and efficient method.It can be used to diagnose,evaluate prognosis,and even guide clinical therapy. |
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| Keywords: | acute myeloid leukemia fluorescence in situ hybridization AML1/ETO fusion gene PML/RARαfusion gene |
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