层黏连蛋白受体反义核酸对卵巢癌细胞侵袭能力的影响

目的:研究67 kD层黏连蛋白受体(67kDLN-R)反义寡核苷酸(antisense oligonucleotide,ASODN)对卵巢癌细胞HRA体外侵袭转移能力的影响.方法:采用流式细胞仪、RT-PCR及Transwell小室的方法检测反义核酸对67 kD LN-R基因和蛋白表达影响,及转染前后细胞体外侵袭转移能力变化.结果:67 kD LN-R ASODN可从蛋白质和mRNA水平下调67kDLN-R的表达,且下调作用呈剂量依赖性.与正义组及对照组相比差异有显著性(P＜0.05).体外侵袭实验证实转染后HRA穿透人工基底膜的能力显著降低并呈现剂量依赖性.结论:67kDLN-R ASODN可降卵巢癌细胞体外侵袭转移能力,有望为卵巢癌治疗提供新方向.

The Inhibitory Effect of 67 kD LN-R Antisense Oligonucleotides on Invasion and Metastasis of Ovarian Carcinoma Cells

Objective; To investigate the effect of 67 kD laminin receptor antisense oligonucleotides (67kD LN-R ASODN) on the invasion and metastasis abilities of ovarian carcinoma cell line HRA. Methods: The ASODN of 67 kD laminin receptor and its control, sense oligonucleotides (SODN) were synthesized and transfected into the target cells. RT-PCR and FCM methods were performed to evaluate the 67 kD LN-R gene and protein expression levels to identify the efficiency of ASODN. The invasiveness of transfected cells was measured quantitatively by matrigel invasion assays (transwell chamber). Results; The 67 kD LN-R gene and protein expression and invasiveness of HRA cells treated with ASODN of different final concentration were significantly decreased compared with that transfected with SODN and the controls(P<0. 05). Conclusion; 67 kD LN-R ASODN has a significant inhibitory effect on the invasiveness of human ovarian carcinoma cell line HRA in a dose-dependent manner. It may become a new gene therapeutic agent for ovarian carcioma.