丙戊酸干预SH-SY5Y细胞的差异蛋白表达

实验采用双向荧光差异凝胶电泳法发现了丙戊酸处理SH-SY5Y细胞有3种差异蛋白表达，这3种蛋白均与细胞内氧化应激有关，其中真核翻译起始因子4A亚基1蛋白和三磷酸腺苷6V亚基1B2亚单位蛋白表达下调，而核内不均一性核糖核蛋白K表达上调。进一步采用基质-辅助激光解析/电离-飞行时间质谱以及蛋白免疫印迹方法鉴定并验证了其中1种蛋白--真核翻译起始因子4A亚基1。说明丙戊酸可通过调节真核翻译起始因子4A亚基1蛋白的表达来进行抗氧化作用。

Valproic acid alters differential protein expression in SH-SY5Y cells

This study sought to identify differentially expressed proteins in SH-SY5Y cells treated with valproic acid, using two-dimensional difference gel electrophoresis analysis. Three proteins were unambiguously identified: the eukaryotic translation initiation factor 4A isoform 1 and ATP6V1B2 protein were downregulated, while the heterogeneous nuclear ribonucleoprotein K was upregulated. Moreover, all three proteins are associated with altered expression due to oxidative stress. Ma-trix-assisted laser desorption/ionization-time of flight mass spectrometry and protein immunoblotting assay confirmed the differential expression of eukaryotic translation initiation factor 4A isoform 1. The results indicate that valproic acid exerts an antioxidation effect by regulating the expression of eukaryotic translation initiation factor 4A isoform 1.